HP1{alpha} targets the chromosomal passenger complex for activation at heterochromatin before mitotic entry

The chromosomal passenger complex (CPC) is directed to centromeres during mitosis via binding to H3T3ph and Sgo1. Whether and how heterochromatin protein 1α (HP1α) influences CPC localisation and function during mitotic entry is less clear. Here, we alter HP1α dynamics by fusing it to a CENP-B DNA-binding domain. Tethered HP1 strongly recruits the CPC, destabilising kinetochore–microtubule interactions and activating the spindle assembly checkpoint. During mitotic exit, the tethered HP1 traps active CPC at centromeres. These HP1-CPC clusters remain catalytically active throughout the subsequent cell cycle. We also detect interactions between endogenous HP1 and the CPC during G2. HP1α and HP1 cooperate to recruit the CPC to active foci in a CDK1-independent process. Live cell tracking with Fab fragments reveals that H3S10ph appears well before H3T3 is phosphorylated by Haspin kinase. Our results suggest that HP1 may concentrate and activate the CPC at centromeric heterochromatin in G2 before Aurora B-mediated phosphorylation of H3S10 releases HP1 from chromatin and allows pathways dependent on H3T3ph and Sgo1 to redirect the CPC to mitotic centromeres.
Source: EMBO Journal - Category: Molecular Biology Authors: Tags: Cell Cycle, Chromatin, Epigenetics, Genomics & Functional Genomics Articles Source Type: research

Related Links:

Publication date: Available online 21 September 2018Source: The Lancet Global HealthAuthor(s):
Source: The Lancet Global Health - Category: International Medicine & Public Health Source Type: research
Head injury is the most common cause of child traumatology. However, there exist no treatment guidelines in children having intracranial lesions due to minor or moderate head trauma. There is little knowledge about monitoring, clinical exacerbation risk fa...
Source: SafetyLit - Category: International Medicine & Public Health Tags: Age: Adolescents Source Type: news
The study examined the insightfulness of mothers who experienced Childhood Sexual Abuse (CSA). Based on attachment theory, we hypothesized that mothers who experienced CSA will be less insightful than those who did not experience CSA, and that state of min...
Source: SafetyLit - Category: International Medicine & Public Health Tags: Age: Adolescents Source Type: news
Gender nonconforming youth are at risk for body dissatisfaction and disordered eating. Currently, only a small body of literature addresses this high-risk group. The five cases in this series highlight important themes for this patient population from an i...
Source: SafetyLit - Category: International Medicine & Public Health Tags: Age: Adolescents Source Type: news
This study assessed the influence of three alcohol-specif...
Source: SafetyLit - Category: International Medicine & Public Health Tags: Age: Adolescents Source Type: news
This study examines whether discrimination based on the body is associated with intentional self-harm and suicidal behavior in adolescence. METHODS: Participants were from the Longitudinal Study of Australian Children (N  = 2948; 48%...
Source: SafetyLit - Category: International Medicine & Public Health Tags: Age: Adolescents Source Type: news
BACKGROUND: Childhood sexual abuse is a common cause of morbidity and mortality. All victims should receive a timely comprehensive medical exam. Currently there is a critical shortage of child abuse pediatricians who can complete the comprehensive child se...
Source: SafetyLit - Category: International Medicine & Public Health Tags: Age: Adolescents Source Type: news
INTRODUCTION: Suicide is one of the leading causes of death among children and young people globally and a major public health issue. Government policies determine how much recognised health issues are prioritised and set the context for investment, develo...
Source: SafetyLit - Category: International Medicine & Public Health Tags: Age: Adolescents Source Type: news
Interparental conflict is detrimental to the development of children. Only few methods for quantifying the degree of interparental conflict exist and this produces controversies about what is detrimental to child well-being and what is not. This is particu...
Source: SafetyLit - Category: International Medicine & Public Health Tags: Age: Adolescents Source Type: news
The primary aim of the study was to investigate the development of psychobehavioral symptoms (internalizing, externalizing, and posttraumatic stress [PTS]) of child welfare-involved adolescents over a 3-year period and to identify predictors of the traject...
Source: SafetyLit - Category: International Medicine & Public Health Tags: Age: Adolescents Source Type: news
More News: