DNA sequencing to help identify crop pests and their natural enemies in agro –ecosystems: the case of the millet head miner Heliocheilus albipunctella (Lepidoptera: Noctuidae) in sub-Saharan Africa

Publication date: Available online 13 March 2018 Source:Biological Control Author(s): Ahmadou Sow, Thierry Brévault, Gérard Delvare, Julien Haran, Laure Benoit, Armelle Coeur d'Acier, Maxime Galan, Cheikh Thiaw, Valérie Soti, Mbacké Sembène Better knowledge of natural enemy communities and their relative contribution to biological control is needed to design ecology-based pest management in agro-ecosystems. Here, we investigated the arthropod communities of pearl millet-based agro-ecosystems in sub-Saharan Africa (Senegal), with a focus on natural enemies of the millet head miner (MHM), Heliocheilus albipunctella (de Joannis). A set of 105 millet fields was monitored over the 2014 to 2016 growing seasons. Arthropods were sampled at three successive phenological stages, from panicle emergence to grain maturity. Eggs and larvae from Lepidoptera pests were collected to assess their identity and relative contribution to the biological control of parasitoids. Morphological identification combined with Sanger and Illumina DNA sequencing, using the COI full barcode (658 bp) and the COI mini-barcode (133 bp) respectively, was carried out to characterize arthropod samples. MHM was by far the main herbivore on millet, with a relative abundance of 97%. Predators were mostly ants (28%), earwigs (Forficula senegalensis, 26%), mirids (20%), spiders (12%), carabid larvae (7%), flower bugs (Orius maxidentex, 6%), and ladybirds (Declivitata hamata, 1%). In all, 13 primary par...
Source: Biological Control - Category: Biology Source Type: research