Combination of resveratrol ‐containing collagen with adipose stem cells for craniofacial tissue‐engineering applications

This study combined collagen scaffolds and human adipose stem cells (hASCs) for oral mucosal and calvarial bone regeneration by using resveratrol (RSV), which affects the differentiation of mesenchymal stem cells. We have evaluated the effect of collagen scaffold‐containing RSV (collagen/RSV) scaffolds both in vitro and in vivo for their wound healing and bone regeneration potential. Scanning electron microscopy and immunostaining results reveal that hASCs adhere well to and proliferate on both collagen scaffolds and collagen/RSV scaffolds. Oral mucosal lesion experiments demonstrated that the collagen/RSV scaffold is more effective in wound closure and contraction than the collagen scaffold. The micro‐computed tomography (μCT) images of calvarial bone display regenerating bone in defects covered with hASCs on collagen/RSV scaffolds that are more visible than that in defects covered with hASCs on a collagen scaffolds. RSV was more effective at inducing hASC differentiation on the collagen scaffold, suggesting that collagen/RSV scaffolds can provide useful biological cues that stimulate craniofacial tissue formation.

Source: International Wound Journal - March 13, 2018 Category: Surgery Authors: Chih ‐Chien Wang, Chih‐Hsin Wang, Hsiang‐Cheng Chen, Juin‐Hong Cherng, Shu‐Jen Chang, Yi‐Wen Wang, Adrienne Chang, Jue‐Zong Yeh, Yi‐Huei Huang, Cheng‐Che Liu Tags: ORIGINAL ARTICLE Source Type: research