Inverse Correlation between Methylation and Expression of the Delta-like Ligands (DLL1) Gene in Gastric Cancer.
This study aimed to explore if the Notch pathway
was abnormally regulated during gastric tumorigenesis. To achieve the goal, Delta-like ligands
(DLL1) gene expression, Notch upstream signal, promoter methylation and its correlation with DLL1
expression were examined by methylation-specific PCR and real time (RT)-PCR in cultured gastric
cancer cell lines or gastric cancer patient samples. Immunostainings and tissue arrays (n = 40) were
used to confirm the DLL1 expression was down-regulated in cancer cells. Transient or stable Notch1
active domain (NICD)-overexpression suppressed proliferation of the gastric cells but the in vivo
tumor growth was enhanced. The results of abnormal DLL1 methylation and expression observed in
early gastric lesions and in gastric cancers may be relevant to the pathogenesis of gastric cancer.
PMID: 29526075 [PubMed - as supplied by publisher]
Source: The Chinese Journal of Physiology - Category: Physiology Tags: Chin J Physiol Source Type: research
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