Geographical discrimination of Glechomae Herba based on fifteen phenolic constituents determined by LC-MS/MS method combined with chemometric methods.
In this study, we developed a strategy integrating targeted analysis and chemometric methods for quality evaluation and discrimination of GH from different geographical origins. Firstly, an accurate and reliable liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed for simultaneous quantification of fifteen phenolic constituents in GH from different geographical origins. The established method was well validated in terms of desirable specificity, linearity, precision and accuracy. Secondly, the quantitative data were subjected to the principal component analysis (PCA) and orthogonal partial least squares discriminant analysis (OPLS-DA). Thirdly, a heatmap visualization was employed for clarifying the distribution of fifteen phenolic compounds in GH from different geographical origins. These results indicated that GH samples from Shandong province have obviously different with those from other provinces in the content of bioactive phenolic constituents. Collectively, the proposed platform might be well-acceptable tool for quality evaluation and discrimination of GH from different geographical origins, providing promising perspectives in tracking the formulation processes of TCMs products.
PMID: 29524341 [PubMed - as supplied by publisher]
Source: Biomedical Chromatography : BMC - Category: Biomedical Science Authors: Tan T, Zhang J, Xu X, Huang WP, Luo Y Tags: Biomed Chromatogr Source Type: research