Reprioritized

You may know I live outside Boston, MA. We had two'little'snow storms in a row. The news is that we lost power from 10pm Wednesday until 7pm Saturday. Nearly 70 hours of now power makes one rethink everything.Yes we have nearly 12 hours of daylight each day. But it seemed to get dark very early each day. The indoor temperature reached 46 degrees yesterday. On the plus side we were relatively well equipped to lose power. We have a gas stove (hot food), gas hot water heater (hot showers), and a fireplace (some warmth). We also have lots of flashlights and a few lanterns and one tiny solar phone charger. The two cats were very snuggly (and bed hogging). But nearly three full days without power is hard.The cause of this power outage was the storm combined with the very wooded neighborhood. There were probably dozens of trees down blocking roads. There were entire telephone poles down across roads that needed to be replaced. There was a big hanging branch sitting on the wires in front of our house. Our neighbors had a tree through their roof. There were many line crews, tree crews, and electrical repair from as far away as Tennessee and Quebec.It was stressful but it made me reprioritize everything. I was not interested in my health. I did not care about my health during that time. I was more concerned with being cold, staying warm, and hoping we did not lose the contents of both the refrigerator and freezer. (I did really miss my heating pad for my back.)Actually, I enjoyed the p...
Source: Caroline's Breast Cancer Blog - Category: Cancer & Oncology Tags: taking care of me Source Type: blogs

Related Links:

In this study, we examined the role of TFEB in TNBC and its relationship with autophagy and DNA damage induced by DOX. In TNBC cells, TFEB was hypo-phosphorylated and localized to the nucleus upon DOX treatment. TFEB knockdown decreased the viability of TNBC cells while increasing caspase-3 dependent apoptosis. Additionally, inhibition of TFEB-phosphatase calcineurin sensitized cells to DOX-induced apoptosis in a TFEB dependent fashion. Regulation of apoptosis by TFEB was not a consequence of altered lysosomal function, as TFEB continued to protect against apoptosis in the presence of lysosomal inhibitors. RNA-Seq analysis...
Source: The Biochemical Journal - Category: Biochemistry Authors: Tags: Biochem J Source Type: research
CONCLUSIONS: Consequently, targeted therapies based on the interaction of PI3K inhibition with BRCA1 mutations or HR deficiency in TNBC may be a promising strategy for the treatment of patients with TNBC. PMID: 31804921 [PubMed - in process]
Source: J Pharm Pharm Sci - Category: Drugs & Pharmacology Authors: Tags: J Pharm Pharm Sci Source Type: research
Targeting bromodomain protein ANCCA/ATAD2 enhances the efficacy of DNA‑damaging chemotherapy agents and radiation. Oncol Rep. 2019 Nov 27;: Authors: Duan Z, Andrews NP, Chen CZ, Fan M, Wang J, Shen J, Li JJ, Chen HW Abstract Bromodomain proteins such as BRD4 chromatin regulator are attractive cancer therapeutic targets. ANCCA (AAA+ nuclear coregulatory cancer‑associated protein, also known as ATPase family AAA domain containing 2 or ATAD2) is a novel oncology drug target and contains a bromodomain and an ATPase domain. Our research group as well as others previously identified ANCCA/ATAD2 as a puta...
Source: Oncology Reports - Category: Cancer & Oncology Tags: Oncol Rep Source Type: research
In this study, we characterized a suite of novel metallated enediyne motifs that usually induced little or no cytotoxicity when two different human cancer cell lines were treated with the compounds at 37°C, but showed a significant enhancement of cytotoxicity after cells were exposed to moderate hyperthermia during drug treatment. Cultured U-1 melanoma or MDA-231 breast cancer cells were treated with various concentrations of Cu, Fe and Zn complexes of the enediyne (Z)-N,N'-bis[1-pyridyl-2-yl-meth-(E)-ylidene]octa-4-ene-2,6-diyne-1,8-diamine (PyED) and clonogenic survival was assessed to determine the effects of the dr...
Source: Radiation Research - Category: Physics Authors: Tags: Radiat Res Source Type: research
Contributors : Christopher J Frost ; Susan C FrostSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensβ-caryophyllene (BCP) exhibits anti-proliferative properties in cancer cells. Here, we examine the hypothesis that BCP induces membrane remodeling. Our data show that high concentrations of BCP increase membrane permeability of human breast cells (hBrC) causing detachment and cell death. At a “ sub-lethal” concentration of BCP, we show that BCP induces a striking upregulation of genes involved in cholesterol biosynthesis, including the gene that encodes for HMGCoA reducta...
Source: GEO: Gene Expression Omnibus - Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Homo sapiens Source Type: research
Contributors : Philip C Schouten ; Daniel J Vis ; Ewald van Dijk ; Esther H Lips ; Esther Scheerman ; Carolien H van Deurzen ; Patricia C Ewing-Graham ; Agnes Jager ; Els M Berns ; Petra M Nederlof ; Lodewyk F Wessels ; Sabine C LinnSeries Type : Genome variation profiling by genome tiling arrayOrganism : Homo sapiensInactivating germline BRCA1 and BRCA2 mutations confer a defect in homologous recombination DNA repair which was found to leave traces in tumor DNA copy number aberration (CNA) profiles. In analogy to previously trained breast cancer CNA classifiers that predicted association with BRCA1 and BRCA2 mutated cance...
Source: GEO: Gene Expression Omnibus - Category: Genetics & Stem Cells Tags: Genome variation profiling by genome tiling array Homo sapiens Source Type: research
Conclusion: : Tumor mutation burdon (TMB) is not associated with the magnitude of the immune response in hereditary BRCA1/2 related breast and ovarian cancers or in sporadic TNBC and sporadic HGSOC. Hereditary tumors express phenotypes as heterogenous as sporadic tumors with various degree of “BRCAness” and various characteristics of the immune microenvironments. The subtyping criteria developed for sporadic tumors can be applied for the classification of hereditary tumors and possibly also characterization of their immune microenvironment. A high HRD score may be a good candidate bi omarker for response to pla...
Source: GEO: Gene Expression Omnibus - Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Homo sapiens Source Type: research
Myokines are peptides known to modulate brain neuroplasticity, adipocyte metabolism, bone mineralization, endothelium repair and cell growth arrest in colon and breast cancer, among other processes. Repeated skeletal muscle contraction induces the production and secretion of myokines, which have a wide range of functions in different tissues and organs. This new role of skeletal muscle as a secretory organ means skeletal muscle contraction could be a key player in the prevention and/or management of chronic disease. However, some individuals are not capable of optimal physical exercise in terms of adequate duration, intens...
Source: Frontiers in Physiology - Category: Physiology Source Type: research
CONCLUSIONS: We identified that 8% of BRCAX patients were carriers of pathogenic variants in genes other than BRCA1 and BRCA2. Therefore, wide gene panels, including clinically actionable genes, should be routinely used in the screening of HBOC in our population. We observed differences from other studies in the prevalence of mutated genes, most likely due to differences in the selection criteria of the probands and in the population analyzed. The high incidence of deleterious variant detection in PALB2 supports its significant role in breast cancer susceptibility and reinforces its inclusion in the HBOC genetic diagnostic...
Source: Clinical Biochemistry - Category: Biochemistry Authors: Tags: Clin Biochem Source Type: research
Authors: Takeshita T, Asaoka M, Katsuta E, Photiadis SJ, Narayanan S, Yan L, Takabe K Abstract Polo-like kinase 1 (PLK1), the most investigated member of the PLK family, plays a pivotal role both in the p53-mediated regulation of DNA damage repair and in mitosis, especially in the G2/M phase. However, the evidence on the clinical and prognostic relevance of PLK1 is limited to triple negative subtype among breast cancer (BC). We hypothesized that high expression of PLK1 is associated with TP53 inactivation, DNA repair deficiency, and worse prognosis in ER positive in BC in a large-scale cohort should clarify its cli...
Source: American Journal of Translational Research - Category: Research Tags: Am J Transl Res Source Type: research
More News: Blogging | Breast Cancer | Cancer | Cancer & Oncology | Gastroschisis Repair | National Institute for Health and Clinical Excelle