GSE111368 Progression of whole blood transcriptional signatures from interferon-induced to neutrophil-associated patterns in patients with severe influenza

Contributors : Jake Dunning ; Simon Blankley ; Long T Hoang ; Mike Cox ; Christine M Graham ; Philip L James ; Chloe I Bloom ; Damien Chaussabel ; Stephen J Brett ; Miriam F Moffatt ; Anne O ’Garra ; Peter J OpenshawSeries Type : Expression profiling by arrayOrganism : Homo sapiensTranscriptional profiles are increasingly used to investigate the severity, subtype and pathogenesis of disease. We now describe whole blood RNA signatures and local and systemic immune mediator levels in a large cohort of adults hospitalised with influenza from which extensive clinical and investigational data was obtained. Signatures reflecting interferon-related antiviral pathways were common up to day 4 of symptoms in cases not requiring mechanical ventilatory support; in those needing mechanical ventilation, an inflammatory, activated neutrophil and cell stress/death ( ‘bacterial’) pattern was seen, even early after disease onset. Identifiable bacterial co-infection was not necessary for this ‘bacterial’ signature but could enhance its development while attenuating the early ‘viral’ signature. Our findings emphasise the importance of timing and severit y in the interpretation of transcriptomic profiles and soluble mediator levels, and identify specific patterns of immune activation that may enable the development of novel diagnostics and therapeutics

Source: GEO: Gene Expression Omnibus - March 3, 2018 Category: Genetics & Stem Cells Tags: Expression profiling by array Homo sapiens Source Type: research