Molecular characterization, toxin detection and resistance testing of human clinical Clostridium difficile isolates from Lebanon.

Molecular characterization, toxin detection and resistance testing of human clinical Clostridium difficile isolates from Lebanon. Int J Med Microbiol. 2018 Feb 22;: Authors: Berger FK, Rasheed SS, Araj GF, Mahfouz R, Rimmani HH, Karaoui WR, Sharara AI, Dbaibo G, Becker SL, von Müller L, Bischoff M, Matar GM, Gärtner B Abstract Clostridium (Clostridioides) difficile is the main cause for nosocomial diarrhoea in industrialised nations. Epidemiologic data on the pathogen's occurrence in other world regions are still scarce. In this context we characterized with phenotypic and molecular genetic methods C. difficile isolates stemming from hospitalised patients with diarrhoea in Lebanon. From 129 stool samples of symptomatic patients at a tertiary care University hospital in Lebanon, a total of 107 C. difficile strains were cultivated and underwent ribotyping, toxin gene detection and antibiotic resistance testing. Ribotype 014 (RT014, 16.8%) predominated, followed by RT002 (9.3%), RT106 (8.4%) and RT070 (6.5%). Binary toxin gene-positive isolates (RT023, RT078 and RT126) were rarely detected and RT027 was absent. Interestingly, within one isolate only the toxin A gene (tcdA) was detected. Multiple-locus variable-number tandem repeat analysis (MLVA) revealed strong strain diversity in most RTs. The isolates were sensitive to metronidazole and vancomycin, and only a small proportion of strains displayed resistance against moxifloxacin, rifampicin, and...
Source: International Journal of Medical Microbiology - Category: Microbiology Authors: Tags: Int J Med Microbiol Source Type: research

Related Links:

No abstract available
Source: Medical Care - Category: Health Management Tags: Special Commentary Source Type: research
Publication date: Available online 11 December 2019Source: The Lancet Global HealthAuthor(s): Adam Wagstaff, Sven NeelsenSummaryBackgroundThe goal of universal health coverage (UHC) requires that everyone receive needed health services, and that families who get needed services do not suffer undue financial hardship. Tracking progress towards UHC requires measurement of both these dimensions, and a way of trading them off against one another.MethodsWe measured service coverage by a weighted geometric average of four prevention indicators (antenatal care, full immunisation, and screening for breast and cervical cancers) and...
Source: The Lancet Global Health - Category: International Medicine & Public Health Source Type: research
Publication date: January 2020Source: The Lancet Neurology, Volume 19, Issue 1Author(s): João Batista Santos Garcia, José Osvaldo Barbosa Neto
Source: The Lancet Neurology - Category: Neurology Source Type: research
Condition:   Chronic Obstructive Pulmonary Disease Intervention:   Behavioral: self-management program Sponsors:   University of Paris 13;   Lebanese University;   Hotel Dieu Hospital Not yet recruiting
Source: - Category: Research Source Type: clinical trials
Condition:   Antibiotic Reaction Intervention:   Diagnostic Test: urine culture Sponsor:   Batool Mutar Completed
Source: - Category: Research Source Type: clinical trials
Microbial Drug Resistance, Ahead of Print.
Source: Microbial Drug Resistance - Category: Microbiology Authors: Source Type: research
Rising antimicrobial resistance (AMR) is a recognised threat to global health. There is a perception that poor prescribing practices contribute to the problem, although data confirming this are limited. Reliable data on the quality of antimicrobial prescribing in UK hospitals are not available, with hospital drug-purchasing data most often being used ...
Source: The Pharmaceutical Journal - Category: Drugs & Pharmacology Source Type: research
Source: International Journal of Tuberculosis and Lung Disease - Category: Respiratory Medicine Authors: Tags: EDITORIALS Source Type: research
Publication date: Available online 10 December 2019Source: The LancetAuthor(s): Maria-Victoria Mateos, Michele Cavo, Joan Blade, Meletios A Dimopoulos, Kenshi Suzuki, Andrzej Jakubowiak, Stefan Knop, Chantal Doyen, Paulo Lucio, Zsolt Nagy, Ludek Pour, Mark Cook, Sebastian Grosicki, Andre Crepaldi, Anna Marina Liberati, Philip Campbell, Tatiana Shelekhova, Sung-Soo Yoon, Genadi Iosava, Tomoaki FujisakiSummaryBackgroundStandard-of-care treatment for patients with newly diagnosed multiple myeloma includes combination therapies for patients who are not eligible for autologous stem-cell transplantation. At the primary analysis ...
Source: The Lancet - Category: General Medicine Source Type: research
Publication date: March 2020Source: Journal of Global Antimicrobial Resistance, Volume 20Author(s): Ana Lucia D. Moro, Maria Helena Rigatto
Source: Journal of Global Antimicrobial Resistance - Category: Infectious Diseases Source Type: research
More News: Antimicrobial Resistance | Avelox | Biaxin | Clarithromycin | Clostridium Difficile | Diarrhoea | Epidemics | Epidemiology | Genetics | Hospitals | Lebanon Health | Metronidazole | Microbiology | Middle East Health | Moxifloxacin