Molecular characterization, toxin detection and resistance testing of human clinical Clostridium difficile isolates from Lebanon.

Molecular characterization, toxin detection and resistance testing of human clinical Clostridium difficile isolates from Lebanon. Int J Med Microbiol. 2018 Feb 22;: Authors: Berger FK, Rasheed SS, Araj GF, Mahfouz R, Rimmani HH, Karaoui WR, Sharara AI, Dbaibo G, Becker SL, von Müller L, Bischoff M, Matar GM, Gärtner B Abstract Clostridium (Clostridioides) difficile is the main cause for nosocomial diarrhoea in industrialised nations. Epidemiologic data on the pathogen's occurrence in other world regions are still scarce. In this context we characterized with phenotypic and molecular genetic methods C. difficile isolates stemming from hospitalised patients with diarrhoea in Lebanon. From 129 stool samples of symptomatic patients at a tertiary care University hospital in Lebanon, a total of 107 C. difficile strains were cultivated and underwent ribotyping, toxin gene detection and antibiotic resistance testing. Ribotype 014 (RT014, 16.8%) predominated, followed by RT002 (9.3%), RT106 (8.4%) and RT070 (6.5%). Binary toxin gene-positive isolates (RT023, RT078 and RT126) were rarely detected and RT027 was absent. Interestingly, within one isolate only the toxin A gene (tcdA) was detected. Multiple-locus variable-number tandem repeat analysis (MLVA) revealed strong strain diversity in most RTs. The isolates were sensitive to metronidazole and vancomycin, and only a small proportion of strains displayed resistance against moxifloxacin, rifampicin, and...
Source: International Journal of Medical Microbiology - Category: Microbiology Authors: Tags: Int J Med Microbiol Source Type: research

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Source: Medical Care - Category: Health Management Tags: Special Commentary Source Type: research
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Source: The Lancet Global Health - Category: International Medicine & Public Health Source Type: research
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Source: The Lancet Neurology - Category: Neurology Source Type: research
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Source: ClinicalTrials.gov - Category: Research Source Type: clinical trials
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Source: ClinicalTrials.gov - Category: Research Source Type: clinical trials
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Source: The Lancet - Category: General Medicine Source Type: research
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Source: Journal of Global Antimicrobial Resistance - Category: Infectious Diseases Source Type: research
More News: Antimicrobial Resistance | Avelox | Biaxin | Clarithromycin | Clostridium Difficile | Diarrhoea | Epidemics | Epidemiology | Genetics | Hospitals | Lebanon Health | Metronidazole | Microbiology | Middle East Health | Moxifloxacin