Fibrinolytic protease from marine Streptomyces rubiginosus VITPSSM.

Fibrinolytic protease from marine Streptomyces rubiginosus VITPSSM. Cardiovasc Hematol Agents Med Chem. 2018 Feb 26;: Authors: Devi CS, Mohanasrinivasan V, Verma PD, Chaterjee S, Keziah SM Abstract Fibrinolytic enzymes must currently originate a significant product in the arena of medical research. Very limited studies are stated on fibrinolyticenzyme production from actinomycetes.Streptomyces sp. isolated from marine soil was chosen to optimize its fibrinolytic protease production. 16s rRNA sequencing confirmed the isolated potent strain to be Streptomyces rubiginosus VITPSSM. A fibrinolytic protease was then purified from Streptomyces rubiginosus VITPSSM, with the target of producing a cost effective feasible enzyme from a potential actinomycete. SDS-PAGE results exhibited a protein band of about 45 kDa and the fibrinolytic band was detected by zymography. Optimization of physical and nutritional parameters for fibrinolytic protease production from a marine soil isolate Streptomyces strain was done by response surface methodology. The optimal cultural condition for fibrinolytic protease production was obtained with response surface methodology was Based on OFAT results, it was inferred that glycerol, Soyabean meal, pH= 7.2 and temperature = 37oC. The optimization of the production of fibrinolytic protease with response surface methodology bring about two folds increase in production by Streptomycesrubiginosus VITPSSM. Thus this stu...
Source: Cardiovascular and Hematological Agents in Medicinal Chemistry - Category: Cardiology Tags: Cardiovasc Hematol Agents Med Chem Source Type: research