Quantitative Peptidomics with Isotopic and Isobaric Tags.

Quantitative Peptidomics with Isotopic and Isobaric Tags.
Methods Mol Biol. 2018;1719:141-159
Authors: Boonen K, De Haes W, Van Houtven J, Verdonck R, Baggerman G, Valkenborg D, Schoofs L
Abstract
In differential peptidomics, peptide profiles are compared between biological samples and the resulting expression levels are correlated to a phenotype of interest. This, in turn, allows us insight into how peptides may affect the phenotype of interest. In quantitative differential peptidomics, both label-based and label-free techniques are often employed. Label-based techniques have several advantages over label-free methods, primarily that labels allow for various samples to be pooled prior to liquid chromatography-mass spectrometry (LC-MS) analysis, reducing between-run variation. Here, we detail a method for performing quantitative peptidomics using stable amine-binding isotopic and isobaric tags.
PMID: 29476509 [PubMed - in process]

Source: Genomics Proteomics ... - February 28, 2018 Category: Genetics & Stem Cells Authors: Boonen K, De Haes W, Van Houtven J, Verdonck R, Baggerman G, Valkenborg D, Schoofs L Tags: Methods Mol Biol Source Type: research