Intracellular TLR7 is activated in human oligodendrocytes in response to Borrelia burgdorferi exposure

Publication date: 3 April 2018 Source:Neuroscience Letters, Volume 671 Author(s): Geetha Parthasarathy, Mario T. Philipp Lyme neuroborreliosis, caused by the gram-negative bacterium Borrelia burgdorferi, may affect the central and/or peripheral nervous systems. In previous studies, we showed that human oligodendrocytes exposed to the bacteria undergo apoptosis in an inflammatory environment, and that inflammatory pathways trigger cell-death pathways. We further demonstrated that several receptor tyrosine kinases were involved in triggering downstream effects, leading to inflammation and apoptosis. Toll-like receptors TLR2 and TLR5, which are commonly studied receptors in Lyme disease, only had a minimal role in inflammatory processes. To delineate the role of other TLRs, if any, real-time RT-PCR array experiments were carried out as an initial screen. Along with several inflammatory genes, TLR7 mRNA was upregulated in cells exposed to B. burgdorferi. Further analysis by immunohistochemistry showed that the TLR7 protein is present in readily detectable amounts, although no discernible differences could be seen between medium and B. burgdorferi-exposed cells by this technique. Nevertheless, use of specific inhibitors and siRNA showed that TLR7 is involved in inducing IL-6 and CCL2 in a dose dependent manner, and likely CXCL8. Triggering an intracellular receptor such as TLR7, which senses RNA, in typically non-phagocytic oligodendrocytes indicates either a niche for the bacter...
Source: Neuroscience Letters - Category: Neuroscience Source Type: research

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Authors: Eldin C, Jaulhac B, Mediannikov O, Arzouni JP, Raoult D Abstract Bacteria of the Borrelia burgdorferi sensu lato complex, responsible for Lyme disease, are members of the spirochetes phylum. Diagnostic difficulties of Lyme disease are partly due to the characteristics of spirochetes as their culture is tedious or even impossible for some of them. We performed a literature review to assess the value of the various diagnostic tests of spirochetes infections of medical interest such as Lyme borreliosis, relapsing fever borreliae, syphilis, and leptospirosis. We were able to draw similarities between these fou...
Source: Medecine et Maladies Infectieuses - Category: Infectious Diseases Tags: Med Mal Infect Source Type: research
This study aimed to investigate the presence and perform molecular characterization of Anaplasmataceae, Rickettsia spp., and Borrelia burgdorferi sensu lato (sl) in tick species collected in Corsica. Ticks from cattle (Bos taurus), sheep (Ovis aries), and rodents (Rattus rattus) were collected from May to September 2016. DNA was purified from ticks, submitted to quantitative real-time polymerase chain reaction (qPCR) and sequenced for phylogenetic analysis. In total, 660 ticks were collected from 111 animals during the study. The most abundant collected tick species from cattle was Rhipicephalus bursa (n = 49...
Source: Ticks and Tick borne Diseases - Category: Zoology Source Type: research
Authors: Boulanger N, Boyer P, Talagrand-Reboul E, Hansmann Y Abstract Ticks are a major group of arthropod vectors, characterized by the diversity of pathogens they transmit, by their impact on human and animal health, and by their socioeconomic implication especially in countries of the Southern Hemisphere. In Europe, Ixodes is the most important tick due to its wide distribution in the ecosystems and the variety of transmitted pathogens, in particular Borrelia (responsible for Lyme borreliosis), but also the tick-borne encephalitis virus. Their increased presence in the environment since the beginning of the 20t...
Source: Medecine et Maladies Infectieuses - Category: Infectious Diseases Tags: Med Mal Infect Source Type: research
(Natural News) A mass migration of birds may be amazing to behold, until they leave countless disease-ridden ticks in their wake. In the U.S., the disease-laden ticks spread in the Northeast and Midwest through dogs which serve as carriers for the Borrelia burgdorferi infection. In 2008, Canadian veterinarians worked on a project which looked into an...
Source: NaturalNews.com - Category: Consumer Health News Source Type: news
Publication date: Available online 6 February 2019Source: International Journal for Parasitology: Parasites and WildlifeAuthor(s): Natalia M. Pukhovskaya, Olga V. Morozova, Nelya P. Vysochina, Nadejda B. Belozerova, Leonid I. IvanovAbstractBorrelia burgdorferi sensu lato (s.l.) DNA was detected by PCR in Ixodes persulcatus Schulze, 1930, Haemaphysalis concinna Koch, 1844, Haemaphysalis japonica douglasi Nuttall et Warburton, 1915 and Dermacentor silvarum Olenev, 1932 ticks collected in the Amur region, the Jewish Autonomous region, the Sakhalin region and on the Khabarovsk territory. Infection rate of I. persulcatus with B...
Source: International Journal for Parasitology: Parasites and Wildlife - Category: Parasitology Source Type: research
Publication date: Available online 4 February 2019Source: Veterinary MicrobiologyAuthor(s): Alena Žákovská, Hana Lya Kučerová, Jiřina Marková, Eva BártováAbstractThe aim of of this study was to determine the seroprevalence of antibodies to B. burgdorferi s.l. in wild small mammals in the Czech Republic and compare sensitivity of PCR and cultivaton. Wild small mammals (n = 691) were trapped in years 2010 – 2014 in three localities of the Czech Republic. Heart rinses (n = 340) and sera (n = 351) were examined by modified indirect ELI...
Source: Veterinary Microbiology - Category: Veterinary Research Source Type: research
CONCLUSION: In the presence of unilateral or bilateral diaphragmatic paralysis of undetermined aetiology, it seems relevant to perform Lyme serology in the blood and, in positive cases, to follow up with a lumbar puncture in order to detect intrathecal IgG synthesis. PMID: 30711345 [PubMed - as supplied by publisher]
Source: Revue des Maladies Respiratoires - Category: Respiratory Medicine Tags: Rev Mal Respir Source Type: research
e;a D, Labruna MB Abstract Microorganisms harbored by Chilean autochthonous ticks have been scarcely studied and current knowledge is restricted to three species of hard ticks only. The current study aimed to assess the presence of Anaplasmataceae, Borrelia and Hepatozoon agents in ticks collected directly from the environment, on reptiles, birds and mammals in twelve localities from northern, central and southern regions of the country and Antarctica. Ticks were identified by means of a morphological and molecular approach. PCR detections point the occurrence of an Anaplasma-like agent and a relapsing fever Borre...
Source: Acta Tropica - Category: Infectious Diseases Authors: Tags: Acta Trop Source Type: research
Borrelia sp. is a causative pathogen of Lyme disease which has become a worldwide health concern. Non-toxic approaches especially directed toward latent persistent forms of this pathogen are desired. Lipids in th...
Source: BMC Complementary and Alternative Medicine - Category: Complementary Medicine Authors: Tags: Research article Source Type: research
Updated Date: Feb 1, 2019 EST
Source: DailyMed Drug Label Updates - Category: Drugs & Pharmacology Source Type: alerts
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