GSE78789 Human NPY/AgRP hypothalamic cell lines for study of obesity susceptibility

Contributors : Li Yao ; Yuanhang Liu ; Iriscilla Ayala ; Zhifang Qiu ; Sateesh Kumar ; Joanne E Curran ; Yidong Chen ; Donna M LehmanSeries Type : Expression profiling by arrayOrganism : Homo sapiensRecent data suggests that common genetic risk for metabolic disorders such as obesity may be human-specific and exert effects through the central nervous system. To overcome the limitation of human tissue access for study, we have generated induced human pluripotent stem cell (hiPSC)- derived neuronal cultures which recapture many features of hypothalamic neurons within the arcuate nucleus. Here we have comprehensively characterized this model across development and benchmarked these neurons to in vivo events. We also demonstrate their utility in study of obesity risk variants. The dynamic transcriptome across neuronal maturation was examined using microarray and RNAseq methods at 9 time points. K-means clustering of the longitudinal data was conducted to identify co-regulation and miRNA control of biological processes. The transcriptomes were compared to those of 103 samples from 13 brain regions reported in the Genotype-Tissue Expression database (GTEx) using principal components analysis. Genes with proximity to body mass index (BMI)-associated genetic variants were mapped to the developmentally expressed genesets, and enrichment significance was assessed with Fisher ’s exact test. The human neuronal cultures have a transcriptional and physiological profile of NPY...
Source: GEO: Gene Expression Omnibus - Category: Genetics & Stem Cells Tags: Expression profiling by array Homo sapiens Source Type: research