GSE98888 Underestimation of inadvertent morpholino RNA targets: evidence from the study of Danio rerio Ser/Arg-rich splicing factors using gene editing tools

Contributors : Marine Joris ; Patrick Motte ; Marc MullerSeries Type : Expression profiling by high throughput sequencingOrganism : Danio rerioWe used morpholinos and gene editing tools to inactivate the srsf5a gene. In contrast to srsf5a homozygous mutants that did not display any phenotypic traits, microinjection of sMOsrsf5a led to developmental defects. By using RNA sequencing on morphants and control embryos we were able to identify a plethora of morpholino inadvertant target.
Source: GEO: Gene Expression Omnibus - Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Danio rerio Source Type: research

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