GSE102873 Genome-wide transcriptome analysis of NIPBL iPSC and commited cardiomyoctes

Conclusion: Our data is the represents the first human developmental model for studying CdLS through pluripotent stem cells and lineage commited subtypes (cardiomyocytes). This data highlights NIPBLs role in regulating transcriptional regulation and has significant consequences on DNA nucleosome involvement as it relates to global gene expression. This work provides preliminary evidence that NIPBL is required for normal epigenetic and DNA landscape establishment during embryonic cardiomyocyte generation.
Source: GEO: Gene Expression Omnibus - Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Homo sapiens Source Type: research

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Publication date: April 2019Source: Biomedicine &Pharmacotherapy, Volume 112Author(s): Zahra Ghanbari Movahed, Mohsen Rastegari-Pouyani, Mohammad hossein Mohammadi, Kamran MansouriAbstractCancer cells can adapt to low energy sources in the face of ATP depletion as well as to their high levels of ROS by altering their metabolism and energy production networks which might also have a role in determining cell fate and developing drug resistance. Cancer cells are generally characterized by increased glycolysis. This is while; cancer stem cells (CSCs) exhibit an enhanced pentose phosphate pathway (PPP) metabolism. Based on ...
Source: Biomedicine and Pharmacotherapy - Category: Drugs & Pharmacology Source Type: research
Publication date: Available online 21 February 2019Source: Stem Cell ReportsAuthor(s): Encarnacion Montecino-Rodriguez, Ying Kong, David Casero, Adrien Rouault, Kenneth Dorshkind, Peter D. PioliSummaryCurrent models propose that reductions in the number of lymphoid-biased hematopoietic stem cells (Ly-HSCs) underlie age-related declines in lymphopoiesis. We show that Ly-HSCs do not decline in number with age. Old Ly-HSCs exhibit changes in gene expression and a myeloid-biased genetic profile, but we demonstrate that they retain normal lymphoid potential when removed from the old in vivo environment. Additional studies ...
Source: Stem Cell Reports - Category: Stem Cells Source Type: research
In this study, we generate isogenic 3D midbrain organoids with or without a Parkinson's disease-associated LRRK2 G2019S mutation to study the pathogenic mechanisms associated with LRRK2 mutation. We demonstrate that these organoids can recapitulate the 3D pathological hallmarks observed in patients with LRRK2-associated sporadic Parkinson's disease. Importantly, analysis of the protein-protein interaction network in mutant organoids revealed that TXNIP, a thiol-oxidoreductase, is functionally important in the development of LRRK2-associated Parkinson's disease in a 3D environment. These results provide proof of princi...
Source: Stem Cell Reports - Category: Stem Cells Source Type: research
Publication date: Available online 21 February 2019Source: Stem Cell ReportsAuthor(s): Sang-Bing Ong, Won Hee Lee, Ning-Yi Shao, Nur Izzah Ismail, Khairunnisa Katwadi, Mim-Mim Lim, Xiu-Yi Kwek, Nathaly Anto Michel, Jiajun Li, Jordan Newson, Soroush Tahmasebi, Jalees Rehman, Kazuki Kodo, Hye Ryoun Jang, Sang-Ging OngSummaryThe relationship between diabetes and endothelial dysfunction remains unclear, particularly the association with pathological activation of calpain, an intracellular cysteine protease. Here, we used human induced pluripotent stem cells-derived endothelial cells (iPSC-ECs) to investigate the effects of dia...
Source: Stem Cell Reports - Category: Stem Cells Source Type: research
Publication date: Available online 21 February 2019Source: Stem Cell ReportsAuthor(s): Stephanie Dooves, Prisca S. Leferink, Sander Krabbenborg, Nicole Breeuwsma, Saskia Bots, Anne E.J. Hillen, Gerbren Jacobs, Marjo S. van der Knaap, Vivi M. HeineSummaryStem cell therapy has great prospects for brain white matter disorders, including the genetically determined disorders called leukodystrophies. We focus on the devastating leukodystrophy vanishing white matter (VWM). Patients with VWM show severe disability and early death, and treatment options are lacking. Previous studies showed successful cell replacement therapy in rod...
Source: Stem Cell Reports - Category: Stem Cells Source Type: research
Publication date: Available online 21 February 2019Source: Stem Cell ReportsAuthor(s): Ah Reum Lee, Kwonho Hong, Seo Hye Choi, Chanhyeok Park, Jae Kyun Park, Jin Il Lee, Jae Il Bang, Dong-Won Seol, Jeoung Eun Lee, Dong Ryul LeeSummaryCryopreservation has a negative effect on the quality of oocytes and may be closely associated with increased levels of reactive oxygen species (ROS) and apoptotic events. The purpose of the present study was to evaluate the detrimental effects on the developmental competence of somatic cell nuclear transferred (SCNT) mouse embryos using vitrified (cryopreserved) oocytes and to evaluate the re...
Source: Stem Cell Reports - Category: Stem Cells Source Type: research
Authors: Mousavi SM, Soroshni S, Hashemi SA, Babapoor A, Ghasemi Y, Mohammad Amani A Abstract At this article, graphene oxide Nano ribbons (GONRs) and its high potential for using in medical fields have been reviewed. Recently, Graphene Nano ribbons (GNRs) has been a field of interest in biological methods and disease treatment such as drug delivery, DNA applications, and photothermal cancer therapies. GNRs demonstrate more efficient properties rather than other graphene-based Nanomaterials due to their larger surface area. These novel properties made them into a remarkable substitute material for biological fields...
Source: Drug Metabolism Reviews - Category: Drugs & Pharmacology Tags: Drug Metab Rev Source Type: research
Karin Kosulin, Herbert Pichler, Anita Lawitschka, Ren é Geyeregger, Thomas Lion
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
Publication date: Available online 21 February 2019Source: The American Journal of SurgeryAuthor(s): Tyler J. Loftus, Elizabeth S. Miller, Jessica K. Millar, Kolenkode B. Kannan, Ines G. Alamo, Philip A. Efron, Alicia M. MohrAbstractBac’kgroundAttenuating post-injury neuroendocrine stress abrogates persistent injury-associated anemia. Our objective was to examine the mechanisms by which propranolol and clonidine modulate this process. We hypothesized that propranolol and clonidine would decrease bone marrow expression of high-mobility group box-1 (HMGB1) and increase expression of stem cell factor (SCF) and B-cell ly...
Source: The American Journal of Surgery - Category: Surgery Source Type: research
Authors: Sun W, Wang Z, Xu Q, Sun H, Liu X, Yang J, Hong R Abstract Cell-based tissue engineering is a promising alternative for periodontal regeneration as current therapies fail to reconstitute tissue damage caused by periodontitis. As newly identified postnatal stem cells, gingiva-derived mesenchymal stem cells (GMSCs) have been focused on for isolation and expansion in vitro of cells with multi-differentiation potential and immunomodulatory capacities. It has been demonstrated that systemically delivered GMSCs can home to the mandibular bone defect sites and promote bone regeneration. However, the role of trans...
Source: Experimental and Therapeutic Medicine - Category: General Medicine Tags: Exp Ther Med Source Type: research
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