An autofluorescence-based method for the isolation of highly purified ventricular cardiomyocytes

ConclusionWe have developed a simple and reliable method for the isolation of highly purified vCMs from the adult murine myocardium, avoiding fixation and permeabilization steps. These isolated vCMs can be used in particular for detailed molecular studies, avoiding contamination with other myocardial cell types.
Source: Cardiovascular Research - Category: Cardiology Source Type: research

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ConclusionCinnamomi Cortex and Scutellariae Radix can prevent the induction of iNOS gene expression, in part through NF ‐κB activation and IL‐1RI upregulation with different mechanisms, indicating that saireito containing both crude drugs may be a potential therapeutic treatment for injured livers.
Source: Traditional and Kampo Medicine - Category: Complementary Medicine Authors: Tags: ORIGINAL ARTICLE Source Type: research
Condition:   Uveal Melanoma, Metastatic Interventions:   Drug: Ipilimumab and nivolumab;   Drug: Melphalan chemosaturation via percutaneous hepatic perfusion Sponsor:   Leiden University Medical Center Recruiting
Source: ClinicalTrials.gov - Category: Research Source Type: clinical trials
Condition:   Heart Failure Intervention:   Procedure: DCD Heart Transplantation Using NRP Sponsor:   NYU Langone Health Recruiting
Source: ClinicalTrials.gov - Category: Research Source Type: clinical trials
In conclusion, common comorbidities in swine result in CMD, in the absence of appreciable atherosclerosis, which is severe en ough to produce perturbations in myocardial oxygen balance, particularly during exercise, resembling key features of INOCA.
Source: Basic Research in Cardiology - Category: Cardiology Source Type: research
Abstract Pulmonary hypertension (PH) is a feature of a variety of diseases and continues to harbor high morbidity and mortality. The main consequence of PH is right-sided heart failure which causes a complex clinical syndrome affecting multiple organ systems including left heart, brain, kidneys, liver, gastrointestinal tract, skeletal muscle, as well as the endocrine, immune, and autonomic systems. Interorgan crosstalk and interdependent mechanisms include hemodynamic consequences such as reduced organ perfusion and congestion as well as maladaptive neurohormonal activation, oxidative stress, hormonal imbalance, a...
Source: Circulation - Category: Cardiology Authors: Tags: Circulation Source Type: research
Cognitive impairment is a common central nervous system complication after heart failure (HF), with an incidence as high as 25-75%.1 Cognitive dysfunction seriously affects patient quality of life and imposes a heavy economic burden on society and families. Many factors are involved in the mechanisms underlying cognitive dysfunction after HF. These factors include low cerebral perfusion, abnormal hormone secretion and the immune/inflammatory response.2,3 Several clinical studies have explored the above damage-inducing mechanisms, but no therapeutic effects have been described.
Source: Journal of Cardiac Failure - Category: Cardiology Authors: Source Type: research
Source: Journal of Nuclear Cardiology - Category: Nuclear Medicine Source Type: research
ConclusionsACR (2-dimensional) and NEMA (3-dimensional) phantoms overestimate partial volume corrections for 1-dimensional LV wall thickness and myocardial perfusion that are corrected with a simple equation that correlates with MACE for optimal risk stratification applicable to most PET-CT scanners for quantifying myocardial perfusion.
Source: Journal of Nuclear Cardiology - Category: Nuclear Medicine Source Type: research
This article reviews the application of an alternative method, functional near-infrared spectroscopy (fNIRS), to the study of cerebral oxygenation changes in MCI and dementia. We synthesized 36 fNIRS studies that examined hemodynamic changes during both the resting state and the execution of tasks of word retrieval, memory, motor control, and visuospatial perception in MCI and dementia. This qualitative review reveals that (amnestic) MCI and AD patients have disrupted frontal and long-range connectivity in the resting state compared to individuals with normal cognition (NC). These patients also exhibit reduced frontal oxyg...
Source: Journal of Psychiatric Research - Category: Psychiatry Source Type: research
Conclusion Both the Intra.Ox and the SPY detected clinically relevant changes in bowel oxygenation following transection and ligation. The use of intravenous ICG dye did not appear to affect measurements of tissue oxygenation obtained using the Intra.Ox. [...] Thieme Medical Publishers 333 Seventh Avenue, New York, NY 10001, USA.Article in Thieme eJournals: Table of contents  |  Abstract  |  Full text
Source: Journal of Reconstructive Microsurgery - Category: Surgery Authors: Tags: Original Article Source Type: research
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