Evaluation of two commercial Real-Time PCR kits, A. fumigatus ® Bio-Evolution and MycoGENIE® A. fumigatus for Aspergillus DNA detection in Bronchoalveolar Lavage Fluid in patients with invasive pulmonary aspergillosis

Publication date: Available online 19 February 2018 Source:The Journal of Molecular Diagnostics Author(s): Julie Denis, Faezeh Forouzanfar, Raoul Herbrecht, Elise Toussaint, Romain Kessler, Marcela Sabou, Ermanno Candolfi, Valérie Letsher-Bru Invasive pulmonary aspergillosis (IPA) is a common complication of immunosuppression. Rapid diagnosis using molecular techniques is essential to improve patient survival. PCR techniques are promising in enhancing Aspergillus detection in blood and respiratory samples. Here, we evaluate for the first time the performances of two commercial Real-Time PCR kits, the A. fumigatus® Bio-Evolution and the MycoGENIE® A. fumigatus for the detection of A. fumigatus DNA in broncho-alveolar lavage (BAL) from patients with and without IPA. Seventy-three BAL were included. Thirty-one of them corresponded to probable IPA patients, 11 to possible IPA and 31 to non-aspergillosis patients, according to the 2008 EORTC/MSG criteria. In the probable IPA group, A. fumigatus ® Bio-Evolution and the MycoGENIE® A. fumigatus Real Time-PCR kits showed 100% of specificity and a sensitivity of 81% and 71%, respectively. The A. fumigatus ® Bio-Evolution detected Aspergillus DNA in the 14 BALs with a positive Aspergillus culture, whereas the MycoGENIE® A. fumigatus PCR was positive only for 12. In the possible IPA group, there was no positive Real Time-PCR, nor positive Aspergillus culture. For the non-aspergillosis patients, no positive result was obs...
Source: The Journal of Molecular Diagnostics - Category: Pathology Source Type: research