In vivo transfer and microevolution of avian native IncA/C2blaNDM-1-carrying plasmid pRH-1238 during a broiler chicken infection study.

In vivo transfer and microevolution of avian native IncA/C2blaNDM-1-carrying plasmid pRH-1238 during a broiler chicken infection study. Antimicrob Agents Chemother. 2018 Feb 05;: Authors: Hadziabdic S, Fischer J, Malorny B, Borowiak M, Guerra B, Kaesbohrer A, Gonzalez-Zorn B, Szabo I Abstract The emergence and spread of carbapenemase-producing Enterobacteriaceae (CPE) in wildlife and livestock animals poses an important safety concern for public health. With our in vivo broiler chicken infection study we investigated transfer and experimental microevolution of the blaNDM-1-carrying IncA/C2 plasmid (pRH-1238) introduced by avian native Salmonella (S.) Corvallis, without inducing antibiotic selection pressure. We evaluated dependency of the time point of inoculation on donor [S Corvallis (12-SA01738)] and a plasmid-free Salmonella spp. recipient [S Paratyphi B (dTa+), 13-SA01617] excretion by quantifying their excretion dynamics. Using S1-PFGE plasmid profiling we gained insight into the variability of native plasmid content among S Corvallis reisolates as well plasmid acquisition in S Paratyphi B (dTa+) and enterobacterial gut microflora. Whole genome sequencing enabled us an in-depth insight into microevolution of pRH-1238 plasmid in S Corvallis and enterobacterial recipient isolates. Our study revealed that the fecal excretion of avian native carbapenemase-producing S Corvallis is significantly higher and not hampered by S Paratyphi...
Source: Antimicrobial Agents and Chemotherapy - Category: Microbiology Authors: Tags: Antimicrob Agents Chemother Source Type: research