Interleukin ‑10 promotes primary rat hepatic stellate cell senescence by upregulating the expression levels of p53 and p21.

Interleukin‑10 promotes primary rat hepatic stellate cell senescence by upregulating the expression levels of p53 and p21. Mol Med Rep. 2018 Feb 12;: Authors: Huang YH, Chen MH, Guo QL, Chen YX, Zhang LJ, Chen ZX, Wang XZ Abstract Liver fibrosis is characterized by the excessive deposition of extracellular matrix (ECM) components, and activated hepatic stellate cells (HSCs) are a primary source of ECM. Several studies have revealed that the induction of HSC senescence may reduce liver fibrosis. The effect of interleukin‑10 (IL‑10) on the senescence of activated HSCs is not fully understood. Therefore, the present study examined its effects and potential mechanisms in activated primary rat HSCs. Collagenase perfusion and density gradient centrifugation methods were used to isolate rat HSCs. HSCs were identified by autofluorescence, Oil Red O staining and immunocytochemical analysis. Activated HSCs were treated with 0, 10, 20 or 40 ng/ml IL‑10 for 24 h. Senescence‑associated β‑galactosidase (SA‑β‑Gal) staining, flow cytometry analysis and a cell counting kit‑8 assay were performed to detect the senescence, apoptosis and viability of rat HSCs, respectively. Reverse transcription‑quantitative polymerase chain reaction, western blot analysis and enzyme linked immunosorbent assays were used to detect the expression of senescence‑associated proteins and cytokines. Freshly isolated rat HSCs exhibited a striking blue...
Source: Molecular Medicine Reports - Category: Molecular Biology Tags: Mol Med Rep Source Type: research