Insight on specificity of uracil permeases of the NAT/NCS2 family from analysis of the transporter encoded in the pyrimidine utilization operon of Escherichia coli

This article is protected by copyright. All rights reserved. RutG is a broad‐specificity uracil/thymine permease encoded in the pyrimidine utilization operon of enterobacteria. An essential difference from the closely related UraA and other uracil‐specific permeases is the absence of a crucial Phe that is predicted to hinder coordination of thymine at the binding site. Replacing this Phe with Ala allows high‐affinity binding and/or transport of thymine in addition to uracil.
Source: Molecular Microbiology - Category: Microbiology Authors: Tags: Research Article Source Type: research