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Frequency Determination of Carbapenem-Resistant Klebsiella Pneumoniae (CRKP) Isolated from hospitals in Isfahan of Iran and Evaluation of Synergistic Effect of Colistin and Meropenem on them.

This study was designed to identification of carbapenemase producing isolates of K. pneumoniae via phenotypic and genotypic methods as well as evaluation of colistin-meropenem combination therapy potential. This study was carried out in Isfahan, of Iran on 100 samples from Alzahra and Khorshid hospitals in 2017. The Modified Hodge Test (MHT) was used to investigate the carbapenemase presence. The minimum inhibitory concentration (MIC) and the Fractional Inhibitory Concentration (FIC) were determined using broth macrodilution and checkerboard assays (respectively) for both meropenem and colistin. The bla-KPC gene was studied by polymerase chain reaction (PCR).The highest and the lowest rate of resistance were observed for piperacillin (84%) and ertapenem (50%) respectively. 68 isolates by MHT were CRKP, but None of them were positive for bla-KPC gene. 21 isolates from CRKP cases were high resistant to used antimicrobial agents in the study that both MIC and FIC results showed significant synergy for this antibiotics in checkerboard test (p-value < 0.05). 21 resistant isolates from CRKP cases showed statistically significant synergy potential for meropenem and colistin. The meropenem-colistin combination therapy can be applied as a suitable antibiotic synergy but it requires further investigation in clinical assay. Regarding to our findings, Probably other mechanisms of resistance to Carbapenems ,except bla-kpc genes are involved. PMID: 29412797 [PubMed - in process]
Source: Cellular and Molecular Biology - Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

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Publication date: April 2018 Source:Microbial Pathogenesis, Volume 117 Author(s): Ahrar Khan, M. Kashif Saleemi, Farah Ali, Muhammad Abubakar, Riaz Hussain, Rao Zahid Abbas, Imtiaz Ahmad Khan Peste des petits ruminants (PPR), an economically important viral transboundary disease of small ruminants is not only prevalent in Pakistan but also in other countries where people rely on agriculture and animal products. The present study was aimed at describing the pathology and antigen localization in natural PPR infections in local (Kajli sheep; Beetal goats) as well as imported small ruminant breeds (Dorper sheep; Australian Bo...
Source: Microbial Pathogenesis - Category: Infectious Diseases Source Type: research
Conditions:   Chlorhexidine;   Ventilator Associated Pneumonia Interventions:   Drug: Chlorhexidine gluconate at 2%;   Drug: Chlorhexidine gluconate at 0.12% Sponsor:   Universidad de Guanajuato Not yet recruiting
Source: ClinicalTrials.gov - Category: Research Source Type: clinical trials
Care of infectious diseases (prevention, diagnosis, and treatment) represents a large part of the activity of pediatric practices as well as primary care, emergency departments, and hospitals. The bacterial and viral species involved in pediatric community-acquired infections (CAIs) can induce severe, moderate, or mild diseases, which require care by hospitalization, emergency departments, or first-line clinicians. For example, pneumococcus manifests as a spectrum of diseases ranging from severe invasive diseases, such as meningitis, bacteremic pneumonia, and bacteremia, to less severe but more frequent diseases, such as a...
Source: The Journal of Pediatrics - Category: Pediatrics Authors: Tags: European Pediatric Association Source Type: research
Conclusion:S. pneumoniae remains a pathogen causing considerable mortality. In adults, non-IPD should be considered of comparable importance as IPD. Increasing pneumococcal vaccine awareness at the healthcare professional and patient levels is essential for increasing vaccine uptake, thus decreasing the incidence, severity and sequelea of pneumococcal disease.
Source: The Journal of Infection in Developing Countries - Category: Infectious Diseases Source Type: research
(University of California - San Diego) Using artificial intelligence and machine learning techniques, researchers at Shiley Eye Institute at UC San Diego Health and University of California San Diego School of Medicine, with colleagues in China, Germany and Texas, have developed a new computational tool to screen patients with common but blinding retinal diseases, potentially speeding diagnoses and treatment.
Source: EurekAlert! - Infectious and Emerging Diseases - Category: Infectious Diseases Source Type: news
Purpose: Fibrotic interstitial lung diseases presenting with nonspecific and overlapping radiologic findings may be difficult to diagnose without surgical biopsy. We hypothesized that baseline quantifiable radiologic features and their short-term interval change may be predictive of underlying histologic diagnosis as well as long-term survival in idiopathic pulmonary fibrosis (IPF) presenting without honeycombing versus nonspecific interstitial pneumonia (NSIP). Materials and Methods: Forty biopsy-confirmed IPF and 20 biopsy-confirmed NSIP patients with available high-resolution chest computed tomography 4 to 24 month...
Source: Journal of Thoracic Imaging - Category: Radiology Tags: Original Articles Source Type: research
Platelet collagen receptor glycoprotein VI (GPVI) and podoplanin receptor C-type lectin-like receptor 2 (CLEC2) are receptors implicated in platelet activation that both signal via an immunoreceptor tyrosine–based activation motif. Platelets are necessary for host defense and prevention of hemorrhage during sepsis, but the role of platelet GPVI and CLEC2 herein is unknown. To investigate this, we infected mice depleted of platelet GPVI or CLEC2 by antibody treatment or GPVI–/– mice with the common human sepsis pathogen Klebsiella pneumoniae via the airways to induce pneumonia-derived sepsis. The GPVI liga...
Source: Blood - Category: Hematology Authors: Tags: Immunobiology and Immunotherapy, Platelets and Thrombopoiesis Source Type: research
Duvelisib is an oral dual inhibitor of phosphoinositide 3-kinase- (PI3K-) and PI3K- in late-stage clinical development for hematologic malignancy treatment. This phase 1 study evaluated maximum tolerated dose (MTD), pharmacokinetics, pharmacodynamics (PD), efficacy, and safety of duvelisib in 210 patients with advanced hematologic malignancies. In the dose escalation phase (n = 31), duvelisib 8 to 100 mg twice daily was administered, with MTD determined as 75 mg twice daily. In the expansion phase (n = 179), patients with indolent non-Hodgkin lymphoma (iNHL), chronic lymphocytic leukemia (CLL), or T-cell lymphoma (TCL) wer...
Source: Blood - Category: Hematology Authors: Tags: Free Research Articles, Lymphoid Neoplasia, Clinical Trials and Observations Source Type: research
ConclusionCaution in utilizing the combination of vancomycin and PTZ is warranted in pediatric patients. Health‐care professionals should be vigilant if this combination is to be initiated, and ensure close monitoring of renal function. Antibiotic therapy de‐escalation should be considered as soon as culture results are available.
Source: Pediatrics International - Category: Pediatrics Authors: Tags: Original Article Source Type: research
Community-acquired pneumonia (CAP) is the leading cause of mortality in children under 5 years of age globally. To improve the management of CAP, we must distinguish CAP from other common pediatric conditions and develop better diagnostic methods to detect the causative organism, so as to best direct appropriate resources in both industrialized and developing countries. Here, we review the diagnostic modalities available for identifying viruses and bacteria in the upper and lower respiratory tract of children, with a discussion of their utility and limitations in diagnosing CAP in children.
Source: Journal of Clinical Microbiology - Category: Microbiology Authors: Tags: Minireviews Source Type: research
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