High temperature requirement factor A1 (HTRA1) regulates the activation of latent TGF- β1 in keloid fibroblasts.

High temperature requirement factor A1 (HTRA1) regulates the activation of latent TGF-β1 in keloid fibroblasts. Cell Mol Biol (Noisy-le-grand). 2018 Jan 31;64(1):107-110 Authors: Jin SF, Wang YX, Xu N, Sun Q, Wang CC, Lv MZ, Sun X, Guo S Abstract Scar treatments are considered a major issue in the plastic surgery field. Activation of the transforming growth factor-β (TGF-β)-mediated signaling pathway plays a key role in the scar pathogeneses, and high temperature requirement factor A1 (HTRA1) inhibits TGF-β1 activation in tumor cells. Our study aims to investigate the role of HTRA1 in the pathogenesis of scars. The mRNA levels of HTRA1 was evaluated by real time PCR, HTRA1 protein expression was determined using western blot and immunohistochemistry, and a luciferase assay was applied to measure dynamic changes of TGF-β1 activity. We found that the expression of HTRA1 was significantly elevated in keloid tissues, compared to normal skin, and TGF-β1 mRNA levels slightly increase in the keloid tissue. Furthermore, active TGF-β1 protein levels and Smad2 phosphorylation significantly increased in the keloid tissue. Treatment with the latent TGF-β1 or recombinant human HTRA1 (rhHTRA1), alone or in combination, increased Smad2 phosphorylation levels in keloid fibroblasts and active TGF-β1 contents of associated supernatants. Our results suggest that HTRA1 is involved in the pathogenesis of scars through regulating ac...
Source: Cellular and Molecular Biology - Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

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Source: Brachytherapy - Category: Cancer & Oncology Authors: Source Type: research
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Source: Brachytherapy - Category: Cancer & Oncology Authors: Source Type: research
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Source: The Journal of Vascular Access - Category: Surgery Tags: J Vasc Access Source Type: research
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Source: Journal of the Medical Association of Thailand = Chotmaihet thangphaet - Category: General Medicine Tags: J Med Assoc Thai Source Type: research
The objective of this study is to investigate the molecular basis of the keloid scar by studying the mutation of related genes. We performed gene screening of mechanoreceptors by quantitative polymerase chain reaction (qPCR), Sanger sequencing to detect the CXCR1gene mutation, and immuno-histochemistry to determine CXCR1 protein expression. Among the genes encoding mechanoreceptors, the expression of CXCR1 mRNA was significantly higher in keloid scar tissues than in the surrounding tissues of normal controls (P   A (p.Gly192Glu). Immunohistochemistry showed heightened protein expression of CXCR1 in keloid sca...
Source: Archives of Dermatological Research - Category: Dermatology Source Type: research
The Journal of Dermatology, EarlyView.
Source: The Journal of Dermatology - Category: Dermatology Authors: Source Type: research
We report an extended cases series of facial skin lumps that were excised using an intra-oral approach.MethodsA retrospective analysis was performed on all patients who underwent excisions of benign cutaneous lesions on the face via an intra-oral approach. Patient demographics, indication for surgery and complications were noted.ResultsForty-seven lesions were excised over a 15-year period. Indications for surgery included benign lesion with minimal skin involvement, risks of keloid/scar hypertrophy and cosmetic factors. Lesions were predominantly epidermoid cysts and lipomas. Mean follow-up was 2.9  years with a 6.4%...
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Efficacy of lentivirus‑mediated Drosophila melanogaster deoxyribonucleoside kinase combined with (E)‑5‑(2‑bromovinyl)‑2'‑deoxyuridine or 1‑β‑D‑arabinofuranosylthymine therapy in human keloid fibroblasts. Mol Med Rep. 2018 May 30;: Authors: Sun Y, Jiang H, Gu M, Zheng X Abstract Keloid scarring is a type of fibroproliferative disease with a high recurrence rate. However, no effective treatment is currently available. Combined therapy with recombinant lentivirus‑mediated Drosophila melanogaster deoxyribonucleoside kinase (Dm‑dNK) and prodrug has been widely studied an...
Source: Molecular Medicine Reports - Category: Molecular Biology Tags: Mol Med Rep Source Type: research
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Source: Polish Journal of Pathology - Category: Pathology Tags: Pol J Pathol Source Type: research
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Source: Aesthetic Plastic Surgery - Category: Cosmetic Surgery Source Type: research
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