A high resolution atmospheric pressure matrix-assisted laser desorption/ionization-quadrupole-orbitrap MS platform enables in situ analysis of biomolecules by multi-mode ionization and acquisition.

In this study, an AP/MALDI source with ultra-high spatial resolution was coupled to a Q Exactive HF orbitrap mass spectrometer for high resolution in situ analysis by MALDI, laserspray ionization (LSI) and matrix assisted ionization (MAI) without instrument modification. LSI and MAI generated multiply charged ions, which expanded the mass detection range and improved fragmentation efficiency. Full MS, targeted MS/MS, data dependent acquisition (DDA) and parallel reaction monitoring (PRM) acquisitions were performed on peptide and protein standards, tissue extracts and tissue sections for in depth characterization of various biomolecules. High resolution full MS and MS/MS images were obtained from crustacean and rat tissues with pixel size less than 30 μm. Overall, AP/MALDI-Q-Orbitrap is a fast scanning instrument that is capable of performing multiple types of ionization and multiple acquisition modes without instrument modification. This instrument platform provides an attractive alternative to other high resolution MALDI instruments. PMID: 29405984 [PubMed - in process]
Source: Analytica Chimica Acta - Category: Chemistry Authors: Tags: Anal Chim Acta Source Type: research

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Authors: Ye MB, Chen YL, Wang Q, An J, Ye F, Jing P Abstract OBJECTIVES: Stroke is a common condition after a transient ischemic attack (TIA) or minor ischemic stroke (IS). Adding clopidogrel to aspirin may yield more beneficial outcomes than aspirin mono-therapy; meanwhile, the risk of bleeding in the acute phase remains poorly understood. Therefore, there is increasing emphasis on the risks and benefits of clopidogrel with aspirin compared with aspirin mono-therapy in an effort to treat TIA/IS. DESIGN: We searched several electronic databases, including PubMed, Cochrane, and Embase, to identify eligible rando...
Source: Scandinavian Cardiovascular Journal - Category: Cardiology Tags: Scand Cardiovasc J Source Type: research
CONCLUSION: Arterial hypertension and elevated diastolic blood pressure are associated with a risk for AS requiring surgery in subjects below 60 years of age. Strict blood pressure control in this group is strongly advocated to avoid other cardiovascular diseases correlated to hypertension. If hypertension and elevated diastolic blood pressure are risk factors for developing AS requiring surgery need further investigations. Notably, elevated fasting glucose levels were related to AS requiring surgery in older adults without concomitant CAD. PMID: 31109205 [PubMed - in process]
Source: Scandinavian Cardiovascular Journal - Category: Cardiology Tags: Scand Cardiovasc J Source Type: research
Authors: Trivino A, Gumireddy A, Meng F, Prasad D, Chauhan H Abstract The major goal of this research was to successfully formulate solid dispersion (SD) of the poorly soluble anticancer drug flutamide (FLT) using various hydrophilic polymers. Furthermore, to get more insight into SD, solid-state studies (miscibility and molecular interaction) were correlated with solution study (precipitation inhibition, dissolution). Hydrophilic polymers like PVP K90, HPMC, Eudragit EPO, and PEG 8000 were used at different drug-to-polymer w/w ratios. Solid-state miscibility studies were carried out using modulated differential sc...
Source: Drug Development and Industrial Pharmacy - Category: Drugs & Pharmacology Tags: Drug Dev Ind Pharm Source Type: research
CONCLUSIONS: Current literature show similar risks for bacterial pathogens. Studies are limited for viral and parasitic pathogens. The evaluated risk-factors for PI-IBS varied among the included studies and the existing evidence is insufficient to identify pathogen-specific risk factors. PMID: 31112663 [PubMed - as supplied by publisher]
Source: Scandinavian Journal of Gastroenterology - Category: Gastroenterology Tags: Scand J Gastroenterol Source Type: research
The Care Quality Commission has observed improvements to culture at an accident and emergency department at the centre of a major bullying scandal.
Source: HSJ - Category: UK Health Source Type: news
Philips Healthcare has garnered U.S. Food and Drug Administration (FDA) 510(k)...Read more on AuntMinnie.comRelated Reading: Philips releases IntraSight interventional app platform Philips' Lumify lands Army airworthiness certification Philips, MIM to collaborate on radiation therapy Philips launches CT, MR simulation scanners at ASTRO Philips launches MRI prostate treatment planning
Source: AuntMinnie.com Headlines - Category: Radiology Source Type: news
Conclusions: Our results revealed that PCSK9 inhibition may moderately improve breast cancer outcomes while having no harmful effects in tumor-bearing mice. PMID: 31110521 [PubMed]
Source: Archives of Medical Science - Category: General Medicine Tags: Arch Med Sci Source Type: research
Accumulating evidence has shown that complicated brain systems are involved in the development and maintenance of chronic low back pain (cLBP), but the association between brain functional changes and clinical outcomes remains unclear. Here, we used resting-state functional magnetic resonance imaging (fMRI) and multivariate pattern analysis to identify abnormal functional connectivity (FC) between the default mode, sensorimotor, salience, and central executive brain networks in cLBP and tested whether abnormal FCs are related to pain and comorbid symptoms. Fifty cLBP patients and 44 matched healthy controls (HCs) underwent...
Source: Pain - Category: Anesthesiology Tags: Research Paper Source Type: research
ConclusionHepatobiliary scintigraphy is a simple, non-invasive, reliable, current and available form for the early study of biliary complications in patients with liver transplantation. Important hepatocellular dysfunction should be taken into account as a frequent cause of false negative studies.ResumenObjetivoMostrar la experiencia del uso de la gammagrafía hepatobiliar en pacientes con sospecha de complicaciones biliares postrasplante hepático en un centro de alta complejidad.Material y métodoEstudio retrospectivo, observacional y descriptivo. Se incluyeron todos los pacientes consecutivos adultos c...
Source: Revista Espanola de Medicina Nuclear e Imagen Molecular - Category: Nuclear Medicine Source Type: research
Publication date: 2019Source: Materials Today: Proceedings, Volume 13, Part 2Author(s): A. Hernández, M.L. Barrios, M.L. SánchezAbstractThis work is focused to study the influence of the reaction media, alkaline or acid media, the annealing temperature, and sonication treatment on size and morphology of silicon dioxide (SiO2) nanoparticles obtained by the sol-gel method. Structural and morphological characterization was performed by: Attenuated total reflectance – Fourier transform infrared (ATR-FTIR) and micro-Raman spectroscopy and scanning electron microscopy (SEM). The FTIR spectrum shows the absorp...
Source: Materials Today: Proceedings - Category: Materials Science Source Type: research
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