Scanning fluorescence correlation spectroscopy comes full circle

Publication date: Available online 7 February 2018 Source:Methods Author(s): German Gunther, David M. Jameson, Joao Aguilar, Susana A. Sánchez In this article, we review the application of fluorescence correlation spectroscopy (FCS) methods to studies on live cells. We begin with a brief overview of the theory underlying FCS, highlighting the type of information obtainable. We then focus on circular scanning FCS. Specifically, we discuss instrumentation and data analysis and offer some considerations regarding sample preparation. Two examples from the literature are discussed in detail. First, we show how this method, coupled with the photon counting histogram analysis, can provide information on yeast ribosomal structures in live cells. The combination of scanning FCS with dual channel detection in the study of lipid domains in live cells is also illustrated.
Source: Methods - Category: Molecular Biology Source Type: research

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Source: Materials Letters - Category: Materials Science Source Type: research
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Source: Materials Science and Engineering: A - Category: Materials Science Source Type: research
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Source: Materials Chemistry and Physics - Category: Materials Science Source Type: research
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