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Scanning fluorescence correlation spectroscopy comes full circle

Publication date: Available online 7 February 2018 Source:Methods Author(s): German Gunther, David M. Jameson, Joao Aguilar, Susana A. Sánchez In this article, we review the application of fluorescence correlation spectroscopy (FCS) methods to studies on live cells. We begin with a brief overview of the theory underlying FCS, highlighting the type of information obtainable. We then focus on circular scanning FCS. Specifically, we discuss instrumentation and data analysis and offer some considerations regarding sample preparation. Two examples from the literature are discussed in detail. First, we show how this method, coupled with the photon counting histogram analysis, can provide information on yeast ribosomal structures in live cells. The combination of scanning FCS with dual channel detection in the study of lipid domains in live cells is also illustrated.
Source: Methods - Category: Molecular Biology Source Type: research

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The objective of the present study was to assess the possible utility of the difference in coronary sinus diameter to quantify stable atherosclerotic plaque(s) using 256-slice coronary computed tomographic angiography. MATERIAL AND METHODS A total of 336 patients were divided into 2 groups with 168 patients each. Patients who had heart failure were included in the study group and those who did not were included in the non-study group. Patients were subjected to cross-sectional study. Cardiovascular images were performed with 256-slice coronary computed tomographic angiography with a prospective electrocardiogram and clinic...
Source: Medical Science Monitor - Category: Research Tags: Med Sci Monit Source Type: research
Authors: Riaz HR, Hashmi SS, Khan T, Hano C, Giglioli-Guivarc'h N, Abbasi BH Abstract Melatonin as plant growth regulator induces differential effects on metabolites that are responsible for reduction, capping and stabilization of zinc oxide nanoparticles. Phytochemical analysis of callus cultures was performed and results were compared with callus cultures supplemented with other plant growth regulators (α-napthalene acetic acid, 2,4-dichlorophenoxy acetic acid and thidiazuron). Highest total phenolic and flavonoid content [42.23 mg of gallic acid equivalent (GAE) g-1 DW and 36.4 mg of (quercet...
Source: Artificial Cells, Nanomedicine and Biotechnology - Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research
Publication date: 1 September 2018 Source:Colloids and Surfaces B: Biointerfaces, Volume 169 Author(s): Yiwen Li, Huailin Yang, Jiuxu Yao, Haiyang Yu, Xin Chen, Peng Zhang, Chunsheng Xiao An amphiphilic biodegradable prodrug (PLG-g-mPEG/CPT) was synthesized by conjugating disulfide-containing camptothecin (CPT) to poly(L-glutamic acid)-graft-methoxy poly(ethylene glycol) (PLG-g-mPEG) through esterification reaction. The amphiphilic prodrugs could self-assemble into micellar nanoparticles and encapsulate doxorubicin (DOX) in aqueous solution at pH 7.4. The treatment of the nanoparticles with reducing glutathione (GSH) at c...
Source: Colloids and Surfaces B: Biointerfaces - Category: Biochemistry Source Type: research
Authors: Brown T, Gutman SA Abstract BACKGROUND: Journals are currently assessed and ranked using a number of different quantitative performance metrics. AIM: To compare and correlate the publication metrics of English-language occupational therapy journals published in 2015. METHOD: Bibliometric data was sourced for 14 English-language occupational therapy journals including the Journal Citations Report (JCR) 2-year impact factor (IF), Eigenfactor Score (EFS), Article Influence Score (AIS), Scopus Source Normalized Impact per Paper (SNIP), Scopus Citescore, and SCImago Journal Rank (SJR) score. The JCR, Sc...
Source: Scandinavian Journal of Occupational Therapy - Category: Occupational Health Tags: Scand J Occup Ther Source Type: research
Authors: Skrzypek TH, Kazimierczak W, Skrzypek H, Valverde Piedra JL, Godlewski MM, Zabielski R Abstract The use of complementary visualization and measurement techniques allowed accurate description and quantification of changes in the intestinal mucosal architecture and provided a comprehensive outlook on the dynamics of remodelling and maturation processes of the mucosal layer taking place in the small intestine of piglets from birth to weaning. The aim of the study was to examine the early postnatal development of the small intestine in pigs. Three techniques were used: scanning electron microscopy (measurement...
Source: Journal of Physiology and Pharmacology - Category: Drugs & Pharmacology Tags: J Physiol Pharmacol Source Type: research
CONCLUSIONS: A radiobiological perspective of meningioma may help to improve clinical results both in terms of tumour control and healthy tissue sparing. Although we are far from drawing any conclusions, this review can lead researchers to identify some cues for future areas of study. PMID: 29775403 [PubMed - as supplied by publisher]
Source: International Journal of Radiation Biology - Category: Radiology Tags: Int J Radiat Biol Source Type: research
Conclusions: Our case demonstrates that histopathological defined diagnosis may significantly inform therapeutic decision-making in irresectable cholangiocarcinoma even in regard to conventional systemic therapy. In case of an undifferentiated carcinoma poly-chemotherapy may provide significant success. PMID: 29774134 [PubMed]
Source: Oncotarget - Category: Cancer & Oncology Tags: Oncotarget Source Type: research
Conclusion: In our study the overlap between baseline and post-therapeutic metabolic tumor sub-volumes was only moderate. These results need to be investigated in a larger cohort acquired with a more standardized patient repositioning protocol for sequential PET imaging. Methods: Pre and post treatment PET/CT scans of ninety four HNSCC patients treated with radio-chemotherapy were retrospectively reviewed. Follow-up 18F-FDG PET/CT images were registered to baseline scans using a rigid body transformation. Seven metabolic tumor sub-volumes were obtained on the baseline scans using a fixed percentage of SUVmax (I30, I40,...
Source: Oncotarget - Category: Cancer & Oncology Tags: Oncotarget Source Type: research
Authors: O'Connell J, Gálvez-González AM, Scandlyn J, Sala-Adam MR, Martín-Linares X Abstract In 2011, the US Department of the Treasury changed its regulations to allow US students to participate in short-term education programs in Cuba. Beginning in 2012, and each year thereafter, Cuba's National School of Public Health and the Colorado School of Public Health have jointly taught a class on the Cuban public health system. The program goals are to provide US students with an opportunity to learn about the Cuban national health system's focus on 1) prevention and primary health care services; 2...
Source: MEDICC Review - Category: International Medicine & Public Health Tags: MEDICC Rev Source Type: research
CONCLUSION: The rare association of bilateral conjunctival granulomas with hypereosinophilic syndrome has been described in this report. PMID: 29771605 [PubMed - as supplied by publisher]
Source: Ocular Immunology and Inflammation - Category: Allergy & Immunology Tags: Ocul Immunol Inflamm Source Type: research
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