Association between Twist and multidrug resistance gene-associated proteins in Taxol ®-resistant MCF-7 cells and a 293 cell model of Twist overexpression.

Association between Twist and multidrug resistance gene-associated proteins in Taxol®-resistant MCF-7 cells and a 293 cell model of Twist overexpression. Oncol Lett. 2018 Jan;15(1):1058-1066 Authors: Wang L, Tan RZ, Zhang ZX, Yin R, Zhang YL, Cui WJ, He T Abstract Multidrug resistance (MDR) severely limits the effectiveness of chemotherapy. Previous studies have identified Twist as a key factor of acquired MDR in breast, gastric and prostate cancer. However, the underlying mechanisms of action of Twist in MDR remain unclear. In the present study, the expression levels of MDR-associated proteins, including lung resistance-related protein (LRP), topoisomerase IIα (TOPO IIα), MDR-associated protein (MRP) and P-glycoprotein (P-gp), and the expression of Twist in cancerous tissues and pericancerous tissues of human breast cancer, were examined. In order to simulate Taxol® resistance in cells, a Taxol®-resistant human mammary adenocarcinoma cell subline (MCF-7/Taxol®) was established by repeatedly exposing MCF-7 cells to high concentrations of Taxol® (up to 15 µg/ml). Twist was also overexpressed in 293 cells by transfecting this cell line with pcDNA5/FRT/TO vector containing full-length hTwist cDNA to explore the dynamic association between Twist and MDR gene-associated proteins. It was identified that the expression levels of Twist, TOPO IIα, MRP and P-gp were upregulated and LRP was downregulated in human breast ca...
Source: Oncology Letters - Category: Cancer & Oncology Tags: Oncol Lett Source Type: research

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Publication date: Available online 15 December 2018Source: Life SciencesAuthor(s): Zhao Ma, Xuebin Bao, Junbao GuAbstractAimFurowanin A (Fur A) is a flavonoid isolated from Millettia pachycarpa Benth. Studies show its potent anti-neoplastic effects against leukemia cells. The aim of the present study was to determine the potential therapeutic effect of Fur A against colorectal cancer (CRC), and elucidate the underlying mechanism.Material and methodsCell Counting Kit-8 (CCK-8) assay was used to determine cell, and TUNEL and Annexin-V/PI staining was used to detect apoptosis and the cell cycle distribution. The expression le...
Source: Life Sciences - Category: Biology Source Type: research
Publication date: Available online 16 December 2018Source: MitochondrionAuthor(s): Justice Afrifa, Tie Zhao, Jingcui YuAbstractThe mitochondria are defined by their unique structure and cellular functions which includes energy production, metabolic regulation, apoptosis, calcium homeostasis, cell proliferation, cell motility and transport as well as free radical generation. Recent advances geared towards enhancing the diagnostic and prognostic value of cancer patients have targeted the circulating mitochondria genome due to its specific and unique characteristics. Circulating mitochondria DNA is known to possess short leng...
Source: Mitochondrion - Category: Biochemistry Source Type: research
Publication date: Available online 16 December 2018Source: The American Journal of SurgeryAuthor(s): Marc Carrier, Alon D. Altman, Normand Blais, Artemis Diamantouros, Deanna McLeod, Uthaman Moodley, Christine Nguyen, Stephanie Young, Frank SchwenterAbstractBackgroundVenous thromboembolism (VTE) includes deep vein thrombosis (DVT) and pulmonary embolism (PE). Certain abdominopelvic cancer surgeries are associated with a six to 14-fold increased risk of DVT versus surgeries for benign disease, and extended thromboprophylaxis using perioperative LMWHs may further reduce VTE rates over standard duration administration. This r...
Source: The American Journal of Surgery - Category: Surgery Source Type: research
Publication date: Available online 15 December 2018Source: Respiratory Medicine Case ReportsAuthor(s): Yang Zhao, Wei GuoAbstractPrimary pulmonary mucosa-associated lymphoid tissue (MALT) lymphoma is extremely rare. MALT lymphoma patients usually show no clinical symptoms or physical signs. Chest radiograph or computed tomography (CT) may confuse MALT lymphoma with other pulmonary diseases, which would lead to misdiagnosis or a delayed diagnosis. In the present study, a 33-year-old male patient had cough and fever. Chest CT showed consolidation on both sides. Those clinical symptoms disappeared after he had been misdiagnos...
Source: Respiratory Medicine Case Reports - Category: Respiratory Medicine Source Type: research
Publication date: Available online 15 December 2018Source: Respiratory Medicine Case ReportsAuthor(s): Keigo Okamoto, Jun HanaokaAbstractCombined subsegmentectomy (CSS) is procedure as diagnostic purpose adding sufficient surgical margin. Compared to other procedure, CSS is not common, with the surgical results also being controversial. We analyzed the patients who had undergoing CSS in the right upper lobe for ground glass opacity (GGO) -dominant nodules. Between April 2011 and March 2017, we experienced 7 cases underwent CSS. In all cases, three subsegments resection and lymph node dissection using Video-Assisted Thoraci...
Source: Respiratory Medicine Case Reports - Category: Respiratory Medicine Source Type: research
Authors: Abstract PMID: 30547738 [PubMed - in process]
Source: Current Cancer Drug Targets - Category: Cancer & Oncology Tags: Curr Cancer Drug Targets Source Type: research
Authors: Sharif K, Coplan L, Lichtbroun B, Amital H Abstract PMID: 30550010 [PubMed - in process]
Source: The Israel Medical Association Journal - Category: General Medicine Tags: Isr Med Assoc J Source Type: research
CONCLUSIONS: These observations suggest that BDNF Val66met-SNP has no detectable effect on the peripheral neuropathy that is induced by paclitaxel. The significance of BDNF Val66Met-SNP in pre-existing peripheral neuropathy-related conditions, such as diabetes, should be further investigated. PMID: 30550003 [PubMed - in process]
Source: The Israel Medical Association Journal - Category: General Medicine Tags: Isr Med Assoc J Source Type: research
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Source: Fight Aging! - Category: Research Authors: Tags: Newsletters Source Type: blogs
CONCLUSION: similar clinical results and quality of life were achieved after treating elderly patients with colon cancer with a multidisciplinary management approach. PMID: 30545228 [PubMed - as supplied by publisher]
Source: Revista Espanola de Enfermedades Digestivas - Category: Gastroenterology Tags: Rev Esp Enferm Dig Source Type: research
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