Cloning, purification, crystallization and preliminary X-ray studies of a carbohydrate-binding module (CBM_E1) derived from sugarcane soil metagenome
In this study, CBM_E1 was expressed, purified and crystallized. X-ray diffraction data were collected to 1.95 Å resolution. The crystals, which were obtained by the sitting-drop vapour-diffusion method, belonged to space group I23, with unit-cell parameters a = b = c = 88.07 Å.
Source: Acta Crystallographica Section F - Category: Biochemistry Authors: Campos, B.M.Alvarez, T.M.Liberato, M.V.Polikarpov, I.Gilbert, H.J.Zeri, A.C.M.Squina, F.M. Tags: accessory domain GH5 carbohydrate-binding module glycoside hydrolases biofuels renewable energy crystallization communications Source Type: research