Influence of the oscillation frequency of different side-to-side toothbrushes on noncontact biofilm removal

ConclusionsThe oscillation frequency of the tested side-to-side toothbrushes affected the biofilm reduction in an interdental space model.Clinical relevanceWithin a toothbrush, higher oscillation frequencies may lead to beneficial effects on interdental biofilm removal by noncontact brushing.
Source: Dental Technology Blog - Category: Dentistry Source Type: news

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In this study, the effects of sub-MICs of certain antibiotics on surface hydrophobicity, cell morphology, and protein profile were ascertained using Fusobacterium nucleatum, Porphyromonas gingivalis and Treponema denticola strains, which are pathogenic bacterial species in periodontal diseases. The MICs of antibiotics were determined by culturing bacteria in media supplemented with serially diluted antibiotic solutions, and sub-MIC of antibiotics was used. The effect of sub-MIC of antibiotics on cell morphology was determined by scanning electron microscopy. Microscopic observation of F. nucleatum and P. gingivalis grown a...
Source: Anaerobe - Category: Microbiology Authors: Tags: Anaerobe Source Type: research
This study investigated some miRNAs and the genes involved in the progression of F. nucleatum-induced colorectal cancer by Affymetrix miRNA microarray technology.We used microarrays to detail the global programme of gene expression underlying cellularisation and identified distinct classes of up-regulated and down-regulated genes between Colorectal cancer and the adjacent normal tissues (CONTROL) infected without F. nucleatum . The miRNA array was performed using the affymetrix GeneChip ® miRNA 3.0 Array (Affymetrix, Santa Clara, California, United States). The chip was processed using a commercial Affymetrix array ser...
Source: GEO: Gene Expression Omnibus - Category: Genetics & Stem Cells Tags: Non-coding RNA profiling by array Homo sapiens synthetic construct Source Type: research
ConclusionsThe amplitude of one of the investigated side-to-side toothbrushes affected the biofilm reduction predictably in an interdental space model.Clinical relevanceWithin certain toothbrushes, a specific amplitude power setting may demonstrate beneficial effects on noncontact biofilm removal.
Source: Clinical Oral Investigations - Category: Dentistry Source Type: research
This study evaluated the antibacterial properties of carvacrol and terpinen-4-ol against Porphyromonas gingivalis and Fusobacterium nucleatum and its cytotoxic effects on fibroblast cells. The minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) were examined. The minimum biofilm inhibition concentration (MBIC) was evaluated by XTT assay. Biofilm decontamination on titanium surfaces was quantified (CFU ml-1), evaluated by confocal laser scanning microscopy (CLSM) and cytotoxic activity by MTT. The MIC and MBC for carvacrol were 0.007% and 0.002% for P. gingivalis and F. nucleatum, and 0.0...
Source: Biofouling - Category: Molecular Biology Authors: Tags: Biofouling Source Type: research
Conclusion LS is well known to specialists in the H&N region, but other disciplines like general surgery, urology, or obstetrics and gynaecology might also rarely encounter the disease. We present a case of LS complicating a perianal abscess that was successfully treated with good outcome.
Source: International Journal of Surgery Case Reports - Category: Surgery Source Type: research
Summary Bacteria residing in oral biofilms live in a state of dynamic equilibrium with one another. The intricate synergistic or antagonistic interactions between them are crucial for determining this balance. Using the six‐species Zürich “supragingival” biofilm model, this study aimed to investigate interactions regarding growth and localization of the constituent species. As control, an inoculum containing all six strains was used, whereas in each of the further five inocula one of the bacterial species was alternately absent, and in the last, both streptococci were absent. Biofilms were grown anaerobi...
Source: Oral Microbiology and Immunology - Category: Microbiology Authors: Tags: ORIGINAL ARTICLE Source Type: research
Hydrogen sulfide (H2S) plays important roles in the pathogenesis of periodontitis. Oral pathogens typically produce H2S from l-cysteine in addition to pyruvate and NH4+. However, fn1055 from Fusobacterium nucleatum subsp. nucleatum ATCC 25586 encodes a pyridoxal 5'-phosphate (PLP)-dependent enzyme that catalyzes the production of H2S and l-serine from l-cysteine and H2O, an unusual cysteine (hydroxyl) lyase reaction (β-replacement reaction). To reveal the reaction mechanism, the crystal structure of substrate-free Fn1055 was determined. Based on this structure, a model of the l-cysteine-PLP Schiff base suggested that ...
Source: Biochemical Journal - Category: Biochemistry Authors: Tags: Research Articles Source Type: research
ConclusionsThe oscillation frequency of the tested side-to-side toothbrushes affected the biofilm reduction in an interdental space model.Clinical relevanceWithin a toothbrush, higher oscillation frequencies may lead to beneficial effects on interdental biofilm removal by noncontact brushing.
Source: Dental Technology Blog - Category: Dentistry Source Type: news
Conclusions: Polystyrene substrates from well plates work as a standard surface and provide reproducible results for in vitro biofilm models. Our biofilm model could serve as a reference point for studies investigating biofilms on different surfaces. PMID: 29535887 [PubMed]
Source: Journal of Periodontal and Implant Science - Category: Dentistry Authors: Tags: J Periodontal Implant Sci Source Type: research
ConclusionsThe oscillation frequency of the tested side-to-side toothbrushes affected the biofilm reduction in an interdental space model.Clinical relevanceWithin a toothbrush, higher oscillation frequencies may lead to beneficial effects on interdental biofilm removal by noncontact brushing.
Source: Clinical Oral Investigations - Category: Dentistry Source Type: research
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