Short hairpin RNA-mediated gene silencing of ADAM17 inhibits the growth of breast cancer MCF ‑7 cells in vitro and in vivo and its mechanism of action.

Short hairpin RNA-mediated gene silencing of ADAM17 inhibits the growth of breast cancer MCF‑7 cells in vitro and in vivo and its mechanism of action. Oncol Rep. 2018 Jan 26;: Authors: Hu B, Meng X, Zhang Y, Hossain MM, Wu L, Zhang Y, Peng X, Zhang X Abstract A disintegrin and metalloprotease 17 (ADAM17) is highly expressed in many malignant tumors and is closely related to their development. We showed in a previous study that silencing of ADAM17 by siRNA inhibited the growth of MCF‑7 breast cancer cells in vitro and in vivo. In the present study, we investigated the effects of ADAM17-short hairpin RNA (ADAM17‑shRNA) on MCF‑7 breast cancer cells and explored the potential action pathway. In vitro, transfection of shRNAs was performed using a lentivirus, and the effects of ADAM17‑shRNA on invasion, proliferation and cell cycle distribution of MCF‑7 cells were assessed by Boyden chamber method, real‑time cell analysis and flow cytometry, respectively. In vivo, MCF‑7 cells with different administrations were transplanted subcutaneously into nude mice, and the effect of ADAM17‑shRNA on the growth of transplanted tumors was assessed. In addition, the morphological structures were observed by H&E staining, and the expression of ADAM17 and Ki‑67 was assessed by immunohistochemistry; expression of ADAM17, EGFR, p‑EGFR, AKT, p‑AKT, ERK and p‑ERK proteins was assessed by western blotting, respectively. Our ...
Source: Oncology Reports - Category: Cancer & Oncology Tags: Oncol Rep Source Type: research