Metabolic response of Aspergillus sydowii to OSMAC modulation produces acetylcholinesterase inhibitors

Publication date: April 2018 Source:Phytochemistry Letters, Volume 24 Author(s): Gesiane da Silva Lima, Aline Mendes da Rocha, Gabriel Franco dos Santos, Alice Ferreira D’Silva, Ivanildo Evódio Marriel, Jacqueline Aparecida Takahashi Fungi are an extraordinary source of bioactive metabolites especially because their metabolic profile can be modulated according to the conditions under which they are grown, a strategy known as OSMAC. Aspergillus sydowii has been showing to be a fungal species with a series of interesting biosynthetic pathways, but production of acethylcholinesterase inhibitors was still not sufficiently explored from this fungal species. In this work, A. sydowii was grown in three different media aiming at awaking biosynthetic routes silent in the works already reported in the literature, in order to produce acethylcholinesterase inhibitors. Using sucrose (150 g/L) and peptone (12 g/L) as sources of carbon and nitrogen respectively, a very active extract was obtained (84.5 ± 2.2% of acethylcholinesterase inhibition). Metabolites 1–5 were isolated from this extract. These metabolites are described from A. sydowii by the first time, to the best of our knowledge. Compound 2 was the most active AChE inhibitor (93.25 ± 0.41%). The results show how small changes in culturing conditions can modulate the production of bioactive secondary metabolites that are silent in other cultivation conditions. Graphical abstract

Source: Phytochemistry Letters - February 3, 2018 Category: Chemistry Source Type: research