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A novel BRCA1 gene deletion detection in human breast carcinoma MCF-7 cells through FRET between quantum dots and silver nanoclusters

Publication date: 15 April 2018 Source:Journal of Pharmaceutical and Biomedical Analysis, Volume 152 Author(s): Yasaman-Sadat Borghei, Morteza Hosseini, Mohammad Reza Ganjali, Saman Hosseinkhani BRCA1 (breast cancer 1) genomic deletions are the most important founder mutations in breast cancer patients and can be passed to you from your mother or father. Herein, we report a silver nanoclusters-based (AgNCs-based) fluorescence resonance energy transfer (FRET) method for detection of BRCA1 gene deletion. The method relies on the specific hybridization of DNA-AgNCs fluorescent probe to deleted genes and interaction between double stranded DNA-AgNCs and QD, and the signal amplification through energy transfer from fluorescent AgNCs to QDs during FRET. Such fabricated QDs/DNA-AgNCs interaction might be beneficial for the nanomaterials based biosensing methods Under best possible conditions a linear correlation was established between the fluorescence intensity and the concentration of deletion sequence in the range of 5.0 × 10−13–1.0 × 10−9 M with a detection limit of 1.2 × 10−13 M. Using this method, we could effectively determine gene deletions by using the nonamplified genomic DNAs that were extracted from the MCF-7 as a breast cancer cell line.
Source: Journal of Pharmaceutical and Biomedical Analysis - Category: Drugs & Pharmacology Source Type: research

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Authors: Vornicova O, Naroditsky I, Boyango I, Shachar SS, Mashiach T, Ilan N, Vlodavsky I, Bar-Sela G Abstract High levels of heparanase are detected in many types of tumors, associated with bad prognosis. Typically, heparanase levels are evaluated in a biopsy taken from the primary lesion, whereas its expression by the resulting metastases is most often unresolved. This becomes critically important as anti-heparanase compounds enter advanced clinical trials. Here, we examined the expression of heparanase in pairs of primary and the resulting distant metastases of breast carcinoma. Interestingly, we found that hep...
Source: Oncotarget - Category: Cancer & Oncology Tags: Oncotarget Source Type: research
Authors: Bronchud MH, Tresserra F, Zantop BS Abstract Microenvironmental properties are thought to be responsible for feto-maternal tolerance. Speculatively, ectopic expression of placental gene programs might also be related to cancer cells' ability to escape from immune vigilance mechanisms during carcinogenesis and cancer progression. Recently, we published the first human genomic evidence of similar immune related gene expression profiles in both placenta (placenta and decidual tissue) and cancer (both primary and metastatic) in the same patient with lymph-node positive breast carcinoma during pregnancy. Here w...
Source: Oncotarget - Category: Cancer & Oncology Tags: Oncotarget Source Type: research
Authors: Vanlandewijck M, Dadras MS, Lomnytska M, Mahzabin T, Lee Miller M, Busch C, Brunak S, Heldin CH, Moustakas A Abstract The multifunctional cytokine transforming growth factor β (TGFβ) controls homeostasis and disease during embryonic and adult life. TGFβ alters epithelial cell differentiation by inducing epithelial-mesenchymal transition (EMT), which involves downregulation of several cell-cell junctional constituents. Little is understood about the mechanism of tight junction disassembly by TGFβ. We found that one of the newly identified gene targets of TGFβ, encoding the serine/th...
Source: Oncotarget - Category: Cancer & Oncology Tags: Oncotarget Source Type: research
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Source: Caroline's Breast Cancer Blog - Category: Cancer & Oncology Tags: breast cancer treatment cancer diagnosis medical errors pathology report Source Type: blogs
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Source: Meta Gene - Category: Genetics & Stem Cells Source Type: research
ConclusionsA small subset of pulmonary adenocarcinomas shows immunoreactivity for ER clones 6F11 and 1D5 in FNA samples (18.2% and 9.1%, respectively). The absence of immunoreactivity for ER‐SP1 clone indicates higher specificity of this clone in non‐breast tissue. The differential diagnostic value of all ER clones in malignant PEs appears to be secure. Larger studies are necessary to validate this observation.
Source: Diagnostic Cytopathology - Category: Pathology Authors: Tags: ORIGINAL ARTICLE Source Type: research
ConclusionThe presence of microcalcifications on imaging and DCIS on initial CNB are associated with residual disease after neoadjuvant chemotherapy in TNBC. These variables can aid in identifying patients with TNBC suitable for inclusion in trials evaluating non‐surgical management after neoadjuvant chemotherapy.
Source: British Journal of Surgery - Category: Surgery Authors: Tags: Original article Source Type: research
CONCLUSION: The presence of microcalcifications on imaging and DCIS on initial CNB are associated with residual disease after neoadjuvant chemotherapy in TNBC. These variables can aid in identifying patients with TNBC suitable for inclusion in trials evaluating non-surgical management after neoadjuvant chemotherapy. PMID: 29465744 [PubMed - as supplied by publisher]
Source: The British Journal of Surgery - Category: Surgery Authors: Tags: Br J Surg Source Type: research
CONCLUSION: CLCA2 suppress NPC proliferation, migration, invasion and epithelial-mesenchymal transition through inhibiting FAK/ERK signaling. PMID: 29463274 [PubMed - in process]
Source: Clinical Genitourinary Cancer - Category: Cancer & Oncology Authors: Tags: J Exp Clin Cancer Res Source Type: research
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Source: Journal of Experimental and Clinical Cancer Research - Category: Cancer & Oncology Authors: Tags: Research Source Type: research
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