GSE80211 Small RNA profiling of WT E14, Ago2_KO and Ago1_KO mouse embryonic stem cells before and after XEN conversion and small RNA profiling after Immunoprecipitation of AGO1 & 2 proteins in WT E14, Ago1_KO and Ago2_KO mESCs.

Contributor : Constance CiaudoSeries Type : Non-coding RNA profiling by high throughput sequencing ; OtherOrganism : Mus musculusPurpose: The goals of this study was to obtain the profile total miRNAs in WT E14, Ago2_KO and Ago1_KO mESCs to compare it with Immunoprecipitated microRNAs in AGO1&2 proteins.
Source: GEO: Gene Expression Omnibus - Category: Genetics & Stem Cells Tags: Non-coding RNA profiling by high throughput sequencing Other Mus musculus Source Type: research

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Publication date: 13 November 2018Source: Cell Reports, Volume 25, Issue 7Author(s): Ayako Nakamura-Ishizu, Takayoshi Matsumura, Patrick S. Stumpf, Terumasa Umemoto, Hitoshi Takizawa, Yuji Takihara, Aled O'Neil, A'Qilah Banu Bte Abdul Majeed, Ben D. MacArthur, Toshio SudaSummaryDuring acute myelosuppression or thrombocytopenia, bone marrow (BM) hematopoietic cells respond rapidly to replenish peripheral blood platelets. While the cytokine thrombopoietin (Thpo) both regulates platelet production and maintains HSC potential, whether Thpo controls megakaryocyte (Mk)-lineage differentiation of HSCs is unclear. Here, we show th...
Source: Cell Reports - Category: Cytology Source Type: research
Publication date: 13 November 2018Source: Cell Reports, Volume 25, Issue 7Author(s): Tao Wu, Yasunao F. Kamikawa, Mary E. DonohoeSummaryThe pluripotent state of embryonic stem cells (ESCs) is defined by its transcriptome and epigenome. The chromatin reader Brd4 determines ESC identity. Although Brd4 regulation in gene transcription has been well described, its contribution to the chromatin landscape is less known. Here, we show that Brd4’s bromodomains partner with the histone acetyltransferase P300, increasing its enzymatic activities. Augmenting histone acetylation by Brd4-P300 interaction recruits the chromatin re...
Source: Cell Reports - Category: Cytology Source Type: research
Publication date: 13 November 2018Source: Cell Reports, Volume 25, Issue 7Author(s): Benoit Souquet, Ellen Freed, Alessandro Berto, Vedrana Andric, Nicolas Audugé, Bernardo Reina-San-Martin, Elizabeth Lacy, Valérie Doye
Source: Cell Reports - Category: Cytology Source Type: research
This study provides biological insights for myogenic differentiation of hPSCs using a double-reporter cell resource and defines an improved myogenic differentiation and purification strategy.Graphical Abstract
Source: Cell Reports - Category: Cytology Source Type: research
Publication date: Available online 13 November 2018Source: Life SciencesAuthor(s): Avinash Kharat, Bhawna Chandravanshi, Shashikant Gadre, Vikrant Patil, Ramesh Bhonde, Aparna DubhashiAbstractAimTo induce differentiation of human amniotic membrane derived mesenchymal stem cells (hAMMSCs) into insulin producing cells (IPCs) by treating with somatocrinin or growth hormone releasing hormone (GHRH) and Insulin-like growth factor-1 (IGF-1).Main methodIn this investigation, we cultivated and characterized hAMMSCs and then treated with IGF-1 and somatocrinin to find out whether this combination gives better yield of insulin produ...
Source: Life Sciences - Category: Biology Source Type: research
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Cardiac progenitor cells (CPCs) being multipotent offer a promising source for cardiac repair due to their ability to proliferate and multiply into cardiac lineage cells. Here, we explored a novel strategy for human CPCs generation from human induced pluripotent stem cells (hiPSCs) using a cardiogenic small molecule, isoxazole (ISX-9) and their ability to grow in the scar tissue for functional improvement in the infarcted myocardium. CPCs were induced from hiPSCs with ISX-9. CPCs were characterized by immunocytochemistry and RT-PCR. The CPC survival and differentiation in the infarcted hearts were determined by in vivo tra...
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