GSE80415 Small RNA profiling of WT E14, Ago2_KO and Ago2_KO-AGO1 mouse embryonic stem cells.

Contributor : Constance CiaudoSeries Type : Non-coding RNA profiling by high throughput sequencingOrganism : Mus musculusPurpose: The goals of this study was to obtain the profile total miRNAs in WT E14, Ago2_KO mESCs to compare with Ago2_KO-AGO1 overexpressing AGO1.
Source: GEO: Gene Expression Omnibus - Category: Genetics & Stem Cells Tags: Non-coding RNA profiling by high throughput sequencing Mus musculus Source Type: research

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Publication date: Available online 21 July 2018Source: Seminars in Cancer BiologyAuthor(s): Jinsong LiuAbstractLife starts with a zygote which is formed via fusion of haploid sperm and egg. The formation of blastomere via cleavage division (nuclear division without increase in cell size) is the first step in the embryogenesis after zygote. The blastomeres are responsible to reprogram parental genome to new embryonic genome for generation of pluripotent stem cells which then differentiate via Waddington’s epigenetic landscape to give a birth for a new life. Multiple authors over the past century and a half have propos...
Source: Seminars in Cancer Biology - Category: Cancer & Oncology Source Type: research
Publication date: Available online 21 July 2018Source: Neurochemistry InternationalAuthor(s): Le Wang, Caitlin R. Schlagal, Junling Gao, Yan Hao, Tiffany J. Dunn, Erica L. McGrath, Javier Allende Labastida, Yongjia Yu, Shi-qing Feng, Shao-yu Liu, Ping WuAbstractHuman neural stem cells (hNSCs) can differentiate into an oligodendrocyte lineage to facilitate remyelination in patients. Molecular mechanisms underlying oligodendrocyte fate specification remains unknown, hindering the development of efficient methods to generate oligodendrocytes from hNSCs. We have found that Neurobasal-A medium (NB) is capable of inducing hNSCs ...
Source: Neurochemistry International - Category: Neuroscience Source Type: research
In this study, we explore the effects of soluble epoxide hydrolase (sEH) deletion on various aspects of adipocyte-function, including programing for white vs. beige-like fat, and mitochondrial and thermogenic gene-expressions. We further hypothesize that EETs and heme-oxygenase 1 (HO-1) form a synergistic, functional module whose effects on adipocyte and vascular function is greater than the effects of sEH deletion alone. In in vitro studies, we examined the effect of sEH inhibitors on MSC-derived adipocytes. MSC-derived adipocytes exposed to AUDA, an inhibitor of sEH, exhibit an increased number of small and healthy adipo...
Source: Prostaglandins and Other Lipid Mediators - Category: Lipidology Source Type: research
miR-140-5p inhibits the proliferation and enhances the efficacy of doxorubicin to breast cancer stem cells by targeting Wnt1, Published online: 22 July 2018; doi:10.1038/s41417-018-0035-0miR-140-5p inhibits the proliferation and enhances the efficacy of doxorubicin to breast cancer stem cells by targeting Wnt1
Source: Cancer Gene Therapy - Category: Cancer & Oncology Authors: Source Type: research
In this study, we tried to demonstrate the effects of adding human umbilical cord blood mesenchymal stem cells (hUCB-MSCs) to carvedilol in improving the doxorubicin- induced cardiotoxicity in rats. Rats were randomly divided into four groups: group 1: control group, group 2: doxorubicin untreated group, group 3: rats injected with doxorubicin and received carvedilol, and group 4: rats injected with doxorubicin and received carvedilol and stem cell-treated. Electrocardiography (ECG) was performed to assess cardiac function after animals were sacrificed. Cardiac muscle sections were examined histologically using H&E, Masson...
Source: Biomedicine and pharmacotherapy = Biomedecine and pharmacotherapie - Category: Drugs & Pharmacology Authors: Tags: Biomed Pharmacother Source Type: research
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Source: Biomedicine and pharmacotherapy = Biomedecine and pharmacotherapie - Category: Drugs & Pharmacology Authors: Tags: Biomed Pharmacother Source Type: research
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Source: Colloids and Surfaces B: Biointerfaces - Category: Biochemistry Source Type: research
This study focused on examining how students’ perception and understanding of the tissue engineering field changed as a result of their participation in a hands-on experiential learning module. The module involved the cellular and biochemical characterization of three-dimensional cultures of mesenchymal stem cells in chondrogenic media. Students were instructed to complete concept maps depicting their perception of the tissue engineering field both prior to and after completion of the module. Similarly, they had to respond to provided design prompts and identify experimental tests and questions for doctors that would...
Source: Education for Chemical Engineers - Category: Chemistry Source Type: research
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Source: Antiviral Therapy - Category: Virology Source Type: research
Contributors : Manlio Vinciguerra ; Tommaso MazzaSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensWe recently described the phenotytpe of HepG2 and Huh-7 hepatocellular carcinoma cells deleted for histone variant macroH2A1, which acquire cancer stem cell phenotype (Lo Re O et al., Hepatology 2017, PMID: 28913935). We found that short hairpin RNA-mediated macroH2A1 knockdown induces acquisition of CSC-like features, including the growth of significantly larger and less differentiated tumors when injected into nude mice. MacroH2A1-depleted HCC cells also exhibited reduced proliferation, ...
Source: GEO: Gene Expression Omnibus - Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Homo sapiens Source Type: research
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