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GSE108871 Systemic post-translational control of bacterial metabolism regulates adaptation in dynamic environments

Contributors : Elizabeth Brunk ; Roger L Chang ; Jing Xia ; Hooman Hefzi ; James T Yurkovich ; Donghyuk Kim ; Evan Buckmiller ; Harris Wang ; Byung-Kwan Cho ; Chen Yang ; Bernhard O Palsson ; George M Church ; Nathan E LewisSeries Type : Expression profiling by arrayOrganism : Escherichia coliE. coli K-12 MG1655 was grown on M9 minimal media supplemented with 0.2% w/v glucose, fructose, or acetate. The goal of this study was to determine differentially expressed genes in the different media conditions.
Source: GEO: Gene Expression Omnibus - Category: Genetics & Stem Cells Tags: Expression profiling by array Escherichia coli Source Type: research

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As the nation’s opioid epidemic rages on, the federal agency charged with leading the government’s response to substance abuse is changing the way it helps local communities. But critics say the move risks leaving programs with fewer resources until the new plan is in place. The controversy began in January with a low-key announcement by the […]Related:E. coli outbreak spreads as source of tainted lettuce remains a mysteryFirst marijuana-derived drug poised for FDA approval after winning support from advisersHave you or someone you know waited too long for an organ transplant?
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In this study, four alginate lyase encoding genes were successfully cloned from Sphingomonas sp. ZH0. The expression systems of these alginate lyases were then constructed in Escherichia coli cells. The recombinant ZH0-I, ZH0-II, ZH0-III and ZH0-IV were purified from E. coli cells and were confirmed to be monomeric enzymes with molecular weights of approximately 91, 52, 67, and 113 kDa, respectively. The conditions for enzymes to have the highest specific lyase activities were 53.2 U/mg, 42 °C, pH 7.0 for ZH0-I, 103.9 U/mg, 47 °C, pH 6.5 for ZH0-II, 13.7 U/mg, 52 °C, pH 7...
Source: Journal of Bioscience and Bioengineering - Category: Biomedical Science Source Type: research
Publication date: 2018 Source:Materials Today: Proceedings, Volume 5, Issue 3, Part 3 Author(s): Sumita Das, P. Sujatha Devi In order create optical platforms for the detection of Escherichia coli (EC) DNA, we have synthesized Cu doped ZnO nanoparticles by a sonochemical method. EC DNA interaction study of the 5% and 10% Cu doped ZnO nanoparticles was monitored by various spectroscopic techniques. Interestingly, 5% Cu doped ZnO shows stronger binding affinity towards EC DNA, but with a lower detection limit of the order of micro molar concentration. The key experimental novelty was observed from the fluorescence titration...
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The aim of this study was to identify the clinical manifestations of cryptosporidiosis and the distribution of Cryptosporidium spp. and subtypes in children in Sonora, Mexico. Two subtypes of C. parvum, including IIaA15G2R1 and IIcA5G3a, and 6 subtypes of Cryptosporidium hominis, including IaA14R3, IaA15R3, IbA12G3, IdA23, IeA11G3T3, and a new subtype IaA14R11, were identified. Cryptosporidium as an etiologic agent for acute gastroenteritis is discussed.
Source: The Pediatric Infectious Disease Journal - Category: Infectious Diseases Tags: Brief Reports Source Type: research
An outbreak of infections linked to bagged romaine lettuce has left salad lovers confused. Here are answers to common questions about leafy greens.
Source: NYT Health - Category: Consumer Health News Authors: Tags: Salads Food Contamination and Poisoning Lettuce Hygiene and Cleanliness Salmonella (Bacteria) Restaurants Agriculture and Farming E Coli (Bacteria) Source Type: news
Public health officials are warning consumers not to eat store-bought, chopped romaine lettuce as an E. coli outbreak linked to the vegetable worsens. Fifty-three related E. coli infections have been reported in 16 states, according to the Centers for Disease Control and Prevention. In the past several days, 18 new cases have been added to the CDC’s investigation, […]Related:First marijuana-derived drug poised for FDA approval after winning support from advisersHave you or someone you know waited too long for an organ transplant?British man who deliberately infected five partners with HIV is sen...
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As of Wednesday 31 people have been hospitalized the 50 have been infected in the outbreak reported in 16 states including Idaho, Pennsylvania, New York and California.
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Fluorescent proteins tend to be toxic, so their clinical applications are sometimes limited and suspect. Researchers from Purdue University and the Korean National Institute of Agricultural Research engineered a new material, made of silk a...
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At least 53 people have been infected so far, and more than half have been hospitalized.
Source: NYT Health - Category: Consumer Health News Authors: Tags: Food Contamination and Poisoning Lettuce E Coli (Bacteria) Centers for Disease Control and Prevention Yuma (Ariz) Source Type: news
The illnesses have been identified as linked to a particularly virulent strain of E. coli O157:H7. Cases have now been reported in five more states, the CDC added, bringing the total number of states involved to 16.
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