Semi-automated set-up for exhaustive micro-electromembrane extractions of basic drugs from biological fluids.

Semi-automated set-up for exhaustive micro-electromembrane extractions of basic drugs from biological fluids. Anal Chim Acta. 2018 Apr 16;1005:34-42 Authors: Dvořák M, Seip KF, Pedersen-Bjergaard S, Kubáň P Abstract Manual handling of microliter volumes of samples and reagents is usually prone to errors and may have direct consequence on the overall performance of microextraction process. Direct connection of a syringe pump and a disposable microextraction unit using flexible polymeric tubing was employed for semi-automated liquid handling in micro-electromembrane extraction (μ-EME). A three-phase μ-EME system was formed by consecutive withdrawal of microliter volumes of donor solution, free liquid membrane (FLM) and acceptor solution into the unit. Excellent repeatability and accuracy of the withdrawal sequence was achieved for solution volumes typically used in μ-EME (1-5 μL) as well as excellent correlation between the initially withdrawn and the finally collected solution volumes. μ-EMEs were initiated by application of d.c. electric potential to the terminal aqueous solutions and specific μ-EME parameters were optimized in order to ensure complete transfer of model analytes from donor to acceptor solution. Exhaustive μ-EMEs of three basic drugs, nortriptyline, papaverine and haloperidol, were achieved from 1.3 μL of acidified donor solution (10 mM HCl) across 2.5 μL of FLM (1-ethyl-2-nitrobenzene) into 1.3â€...
Source: Analytica Chimica Acta - Category: Chemistry Authors: Tags: Anal Chim Acta Source Type: research