An improvised medium for axenic cultivation of Acanthamoeba spp.

Kirti Megha, Amit Gupta, Rakesh Sehgal, Sumeeta KhuranaIndian Journal of Medical Microbiology 2017 35(4):597-599Acanthamoebae can be easily grown in bacterised cultures, but their growth in axenic media is tedious and many times unsuccessful. We thus experimented with some additives in the conventional axenic medium for growth of various isolates of Acanthamoeba. Addition of Torula yeast RNA was found to significantly enhance the growth of Acanthamoebae in the axenic culture medium.
Source: Indian Journal of Medical Microbiology - Category: Microbiology Authors: Source Type: research