Detecting endomysial and tissue transglutaminase antibodies in patients with giardiasis

AbstractCeliac disease is a genetic disease diagnosed to be associated with chronic intestinal inflammation. Endomysial and tissue transglutaminase antibodies are the best serum markers for celiac disease (CD) diagnosis. This research aimed to determine the levels of endomysial and tissue transglutaminase antibodies in patients with giardiasis. Forty cases of giardiasis were selected among the referees to Milad Hospital in Tehran, as well as a children ’s hospital and some health centers in Karaj. Euro-immune IgA immunofluorescence and ELISA were utilized to measure their titers of endomysial and tissue transglutaminase antibodies, respectively. Twenty random serum samples of negativeGiardia and 16 serum samples of positive anti-(tissue transglutaminase) tTG antibodies were evaluated by using ELISA and endomysium antibody through immunofluorescence techniques. Among the 40 cases of giardiasis, 16 positive endomysial and transglutaminase antibodies (40%) were detected. Sixteen positive samples of endomysial antibody (EMA) were also positive for anti-tTG, and 20 random negative samples were negative for EMA and anti-tTG. The chi-square test revealed a significant association between antibodies and giardiasis (P = 0.001). Atrophy of the intestinal villi can arise after giardia infection by mimicking the behavioral patterns of anti-EMA and anti-tTG antibodies. Due to low specificity, anti-gliadin antibody test is not helpful in detecting CD in patients with giardiasis. In t...
Source: Comparative Clinical Pathology - Category: Pathology Source Type: research