Derivation of human induced pluripotent stem cell line (HUSTi001-A) from a 40-year-old patient with idiopathic infertility

Publication date: March 2018 Source:Stem Cell Research, Volume 27 Author(s): Fang Fang, Zili Li, Qian Zhao, Zhen Ye, Kai Zhao, Xiuli Gu, Shiliang Shen, Renee A. Reijo Pera, Chengliang Xiong Human fibroblasts were isolated from foreskin of a clinically diagnosed 40-year old patient with idiopathic infertility. The fibroblasts were reprogrammed with the Yamanaka KOSM transcriptional factors using the retroviral vectors. The obtained induced pluripotent stem cell (iPSC) line showed pluripotency verified by the expression of pluripotency markers, NANOG, SOX2, OCT4, TRA-1-60, and SSEA-4. And the iPSC line was demonstrated to have the three germ layers differentiation capacity in vivo by teratoma assay. The iPSC line also showed normal karyotype. This patient-specific iPSC line can be used to explore the mechanism for idiopathic male infertility.
Source: Stem Cell Research - Category: Stem Cells Source Type: research