GSE109714 Retinal Cell Type Epigenetic Memory Predicts Reprogramming Efficiency and Retinogenesis in 3D Organoid Cultures [TEBS_Mm]

Contributors : Lu Wang ; Daniel Hiler ; Beisi Xu ; Issam Aldiri ; Xiang Chen ; Lyra Griffiths ; Marc Valentine ; Abbas Shirinifard ; Andras Sablauer ; Suresh Thiagarajan ; Marie-Elizabeth Barabas ; Jiakun Zhang ; Dianna Johnson ; Sharon Frase ; Michael A DyerSeries Type : Methylation profiling by high throughput sequencingOrganism : Mus musculusDiverse cell types can be reprogrammed into pluripotent stem cells by ectopic expression of Oct4 (Pou5f1), Klf4, Sox3 and Myc. Many of these induced pluripotent stem cells (iPSCs) retain an epigenetic memory of their cellular origins and this in turn may bias their subsequent differentiation. Differentiated neurons are difficult to reprogram and there has not been a systematic side-by-side characterization of reprogramming efficiency or epigenetic memory across different neuronal subtypes. We have recently developed a new method for reprogramming retinal neurons and successfully reprogrammed rod photoreceptors from the murine retina. Here we extended our retinal reprogramming to cone photoreceptors, bipolar neurons, amacrine/horizontal cell interneurons and M üller glia at two different stages of development. We scored the efficiency of reprogramming across all 5 retinal cell types at each developmental stage and we measured retinal differentiation from each iPSC line using a quantitative standardized scoring system called STEM-RET. We discovered that t he rod photoreceptors and bipolar neurons had the lowest reprogrammi...
Source: GEO: Gene Expression Omnibus - Category: Genetics & Stem Cells Tags: Methylation profiling by high throughput sequencing Mus musculus Source Type: research