Distribution of Fos-immunoreactive neurons in the gustatory cortex elicited by intra-oral infusion of taste solutions in conscious rats.

Distribution of Fos-immunoreactive neurons in the gustatory cortex elicited by intra-oral infusion of taste solutions in conscious rats.
Brain Res. 2018 Jan 22;:
Authors: King MS
Abstract
The location of neurons in the gustatory cortex (GC) activated by intra-oral infusion of solutions in conscious rats was mapped using Fos immunohistochemistry. Groups of adult male Wistar rats (N's=5) received an infusion of one of the following: dH2O, 0.1 or 1.0M NaCl, 0.1 or 1.0M sucrose, 0.32M MSG (with 100µM amiloride and 2.5M inosine 5'-monophosphate), 0.03M HCl, or 0.003M QHCl delivered via an intra-oral cannula (0.233ml/min for 5 min). Unstimulated control rats received no infusion. Taste reactivity (TR) behaviors were videotaped and scored. The number of Fos-immunoreactive (Fos-IR) neurons was counted in eight sections throughout the anterior-posterior extent of the GC in the medial and lateral halves of the granular (GI), dysgranular (DI), and dorsal (AID) and ventral (AIV) agranular insular cortices. Intra-oral infusion of dH2O, NaCl, or sucrose altered the number of Fos-IR neurons in only specific subareas of the GC and the effects of these tastants were concentration-dependent. For example, 1.0M NaCl increased Fos-IR neurons in the posterior lateral AID and DI and elicited more aversive TR responses than 0.1M NaCl. Compared to dH2O, infusions of HCl or QHCl increased the total number of Fos-IR neurons in many subareas of the GC througho...

Source: Brain Research - January 22, 2018 Category: Neurology Authors: King MS Tags: Brain Res Source Type: research