Xylanase B from Clostridium cellulovorans 743B: overexpression, purification, crystallization and X-ray diffraction analysis
In this study, XynB was cloned, overexpressed, purified and crystallized. XynB was crystallized using the hanging-drop vapour-diffusion method in the presence of 0.2 M sodium acetate trihydrate, 0.1 M Tris – HCl pH 8.5, 32%(w/v) PEG 4000 at 293 K. X-ray diffraction analysis revealed that the crystal diffracted to 1.95 Å resolution and belonged to space group P212121, with unit-cell parameters a = 74.28, b = 77.55, c = 88.20 Å , α = β = γ = 90 ° . The data-evaluation statistics revealed high quality of the collected data, thereby establishing a solid basis for determination of the structure of cellulosomal xylanase from C. cellulovorans.
Source: Acta Crystallographica Section F - Category: Biochemistry Authors: Nakajima, D. Nagano, A. Shibata, T. Tanaka, R. Kuroda, K. Ueda, M. Miyake, H. Tags: xylanases xylanase B Clostridium cellulovorans cellulosome CBM family 22 glycoside hydrolase family 10 research communications Source Type: research