Enhanced Tissue Clearing Solution, Clearing-Enhanced 3D (Ce3D), Compatible with Advanced Fluorescence Microscopy Imaging

NIH immunologists have created a solution, Clearing-enhanced 3D (Ce3D), that can be used to make entire organs extremely transparent (top right panel). This allows the tissue to be imaged using advanced fluorescence microscopy techniques (bottom panel). Unlike current tissue clearing solutions, the Ce3D tissue clearing solution is robustly compatible with a variety of staining methods, and preserves tissue morphology and reporter fluorescence. Ce3D enabled microscopy provides unprecedented insight into the spatial organization of cells within intact organs. Further, when Ce3D enabled microscopy is coupled with multiplexed staining and a newly developed analysis pipeline, investigators are able to extensively characterize densely packed cells in situ, providing advantages to phenotyping cells with flow cytometric techniques.IC: NIAIDNIH Ref. No.: E-168-2016/0Advantages: Simple, quick and inexpensive procedure that has been extensively validatedGenerates excellent tissue transparency, resulting in high quality imagesCompatible with highly multiplexed staining/labeling techniquesFluorescence is maintained in diverse fluorescent proteins and fluorophoresEnables quantitative analysis of tissue composition and cellular distribution in whole organs, and has advantages over flow cytometric techniquesApplications: Research reagent – can be applied to a variety of biological disciplinesDiagnostic medical imaging reagent – characterization of disease state/condit...
Source: NIH OTT Licensing Opportunities - Category: Research Authors: Source Type: research