Emerging discrepancies in conventional and molecular epidemiology of methicillin resistant Staphylococcus aureus isolated from bovine milk

Publication date: March 2018 Source:Microbial Pathogenesis, Volume 116 Author(s): Amjad Islam Aqib, Muhammad Ijaz, Shahid Hussain Farooqi, Rais Ahmed, Muhammad Shoaib, Muhammad Muddassir Ali, Khalid Mehmood, Hui Zhang Methicillin resistant Staphylococcus aureus (MRSA) is an emerging public health concern from dairy milk, and its diagnosis by phenotypic methodology is experiencing higher discrepancies. The present study was planned to estimate discrepancies in phenotypic identification of MRSA and MSSA (Methicillin sensitive Staphylococcus aureus) in relation to mecA, and prevalent risk factors from various localities. In-vitro oxacilline antibiotic disks were used for phenotypic identification of MRSA, whereas mecA gene was used as MRSA marker in Staph aureus by PCR. Total of 900 bovine milk samples from private and public farms located in district Faisalabad using convinent sampling technique were collected. Potential risk factors for MRSA prevalence identified by non-parametric statistical tests were compared among different subdistricts. Discrepancy in MRSA was calculated as percentage of mecA negative strains while that of MSSA was determined as percentage of mecA positive strains. Molecular identification presented 17.97% (55/306) of discrepancy in MRSA in terms of negative mecA strains from district Faisalabad while sub-district Faisalabad, sub-district Jaranwala, and sub-district Samundary presented 13.98% (13/93), 18.28% (17/93), and 20.83% (25/120) discrepa...
Source: Microbial Pathogenesis - Category: Infectious Diseases Source Type: research