Validation of Anti-CSP α, SNAP25, Tyrosine Hydroxylase, Ubiquitin, Cleaved Caspase 3, and pSer PKC Motif Antibodies for Utilization in Western Blotting.

In this study, we validated several commercial antibodies, including anti-CSPα, SNAP25, tyrosine hydroxylase, ubiquitin, cleaved caspase 3, and pSer PKC motif. Anti-CSPα, SNAP25, and tyrosine hydroxylase antibodies could detect their endogenous target proteins with some degree of cross-reactivity. Furthermore, clear SNAP25 staining was observed with SNAP25 antibody. Antibodies directed against ubiquitin, cleaved caspase 3, and pSer PKC motif could detect poly-ubiquitination, apoptosis, and phosphorylation, respectively.
PMID: 29343881 [PubMed]

Source: Acta Histochemica et Cytochemica - December 26, 2017 Category: Biochemistry Authors: Shirafuji T, Ueyama T, Tanaka S, Hide I, Saito N, Sakai N Tags: Acta Histochem Cytochem Source Type: research