Isolation, molecular characterization and antimicrobial resistance of enterobacteriaceae isolated from fish and seafood

Publication date: June 2018 Source:Food Control, Volume 88 Author(s): Amira Leila Dib, Amir Agabou, Amina Chahed, Cemil Kurekci, Elena Moreno, Miguel Espigares, Elena Espigares This current study attempted to examine the occurrence rates of Enterobacteriaceae in seafood samples collected from different fishmongers in the province of Constantine (Northeast Algeria) over a one-year period. Total and fecal coliforms were enumerated and selected Enterobacteriaceae colonies were identified. Escherichia coli (E. coli) and Salmonella isolates were serotyped, after which their antimicrobial susceptibility patterns were determined. Extended-spectrum beta-lactamases (ESBL) encoding genes, virulence genes (stx1, stx2 and eae) and plasmid-harboring were searched among E. coli isolates and their clonality was also assessed by pulsed-field gel electrophoresis (PFGE). Results showed that fecal coliform contamination levels are beyond the recommended limits. All strains of Salmonella (n = 2) isolated were of serovar infantis. One E. coli isolate from sardines belonged to serotype O127 and two others (one from sardines and one from shrimps) were identified as serotype O125. One E. coli isolate from sardines was characterized as enterohemorrhagic (EHEC) and six others as shiga toxin-producing (STEC) (three from sardines and three from shrimps). All tested strains were multidrug resistant, with five E. coli strains showing an ESBL phenotype and harboring the bla CTX-M-15 gene ...
Source: Food Control - Category: Food Science Source Type: research