Conditionally reprogrammed cells (CRC) methodology does not allow the in vitro expansion of patient ‐derived primary and metastatic lung cancer cells

In conclusion, patient‐derived primary and metastatic lung cancer cells were negatively selected under CRC conditions, limiting the expansion to non‐malignant lung epithelial stem cells either from tumor and non‐tumor tissue sources. Thus, CRC approach cannot be applied for direct therapeutic testing of patient lung tumor cells, as the tumor‐derived CRC cultures are composed of (non tumoral) airway basal cells. This article is protected by copyright. All rights reserved.

Source: International Journal of Cancer - January 17, 2018 Category: Cancer & Oncology Authors: Giovanni Sette, Valentina Salvati, Ilenia Giordani, Emanuela Pillozzi, Denise Quacquarini, Enrico Duranti, Francesca De Nicola, Matteo Pallocca, Maurizio Fanciulli, Mario Falchi, Roberto Pallini, Ruggero De Maria, Adriana Eramo Tags: Research Article Source Type: research