A Novel l-Glutamate Exporter of Corynebacterium glutamicum.

A Novel l-Glutamate Exporter of Corynebacterium glutamicum. Appl Environ Microbiol. 2018 Jan 12;: Authors: Wang Y, Cao G, Xu D, Fan L, Wu X, Ni X, Zhao S, Zheng P, Sun J, Ma Y Abstract Besides metabolic pathways and regulatory networks, transport systems are also pivotal for cellular metabolism and hyper-production of biochemicals using microbial cell factories. Identification and characterization of transporters are therefore of great significance for understanding and engineering of transport reactions. Herein, a novel l-glutamate exporter MscCG2 that extensively exists in Corynebacterium glutamicum strains but is distinct from the only known l-glutamate exporter MscCG was discovered in an industrial l-glutamate producing C. glutamicum MscCG2 was predicted to possess three transmembrane helices in the N-terminal region and located in the cytoplasmic membrane, which are typical structural characteristics of the mechanosensitive channel of small conductance. MscCG2 has a low amino acid sequence identity (23%) to MscCG and evolved separately from MscCG with four transmembrane helices. Despite the considerable differences between MscCG2 and MscCG in sequence and structure, gene deletion and complementation confirmed that MscCG2 also functioned as an l-glutamate exporter and an osmotic safety valve in C. glutamicum Besides, transcriptional analysis showed that MscCG2 and MscCG genes were transcribed in similar patterns and not induced b...
Source: Applied and Environmental Microbiology - Category: Microbiology Authors: Tags: Appl Environ Microbiol Source Type: research