Quantitative Analysis Of Lipid Debris Accumulation Caused By Cuprizone Induced Myelin Degradation In Different Cns Areas.

Quantitative Analysis Of Lipid Debris Accumulation Caused By Cuprizone Induced Myelin Degradation In Different Cns Areas. Brain Res Bull. 2018 Jan 08;: Authors: Ozsvár A, Szipőcs R, Ozsvár Z, Baka J, Barzó P, Tamás G, Molnár G Abstract Degradation of myelin sheath is thought to be the cause of neurodegenerative diseases, such as multiple sclerosis (MS), but definitive agreement on mechanism of how myelin is lost is currently lacking. Autoimmune initiation of MS has been recently questioned by proposing that the immune response is a consequence of oligodendrocyte degeneration. To study the process of myelin breakdown, we induced demyelination with cuprizone and applied coherent anti-Stokes Raman scattering (CARS) microscopy, a non-destructive label-free method to image lipid structures in living tissue. We confirmed earlier results showing a brain region dependent myelin destructive effect of cuprizone. In addition, high resolution in situ CARS imaging revealed myelin debris forming lipid droplets alongside myelinated axon fibers. Quantification of lipid debris with a custom-made software for segmentation and three dimensional reconstruction revealed brain region dependent accumulation of lipid drops inversely correlated to the thickness of myelin sheaths. Finally, we confirmed that in situ CARS imaging is applicable to living human brain tissue in brain slices derived from a patient. Thus, CARS microscopy is potent tool for qua...
Source: Brain Research Bulletin - Category: Neurology Authors: Tags: Brain Res Bull Source Type: research