Stability, biocompatibility and antioxidant activity of PEG-modified liposomes containing resveratrol

Publication date: 1 March 2018 Source:International Journal of Pharmaceutics, Volume 538, Issues 1–2 Author(s): Carla Caddeo, Laura Pucci, Morena Gabriele, Claudia Carbone, Xavier Fernàndez-Busquets, Donatella Valenti, Ramon Pons, Antonio Vassallo, Anna Maria Fadda, Maria Manconi The present investigation reports the development of PEG-modified liposomes for the delivery of naturally occurring resveratrol. PEG-modified liposomes were prepared by direct sonication of the phospholipid aqueous dispersion, in the presence of two PEG-surfactants. Small, spherical, unilamellar vesicles were produced, as demonstrated by light scattering, cryo-TEM, and SAXS. The aging of the vesicles was assessed by using the Turbiscan® technology, and their physical stability was evaluated in vitro in simulated body fluids, results showing that the key features of the liposomes were preserved. The biocompatibility of the formulations was demonstrated in an ex vivo model of hemolysis in human erythrocytes. Further, the incorporation of resveratrol in PEG-modified liposomes did not affect its intrinsic antioxidant activity, as DPPH radical was almost completely inhibited, and the vesicles were also able to ensure an optimal protection against oxidative stress in an ex vivo human erythrocytes-based model. Therefore, the proposed PEG-modified liposomes, which were prepared by a simple and reliable method, represent an interesting approach to safely deliver resveratrol, ensuring the prese...
Source: International Journal of Pharmaceutics - Category: Drugs & Pharmacology Source Type: research