VEGF siRNA delivery by a cancer-specific cell-penetrating peptide.

In this study, we investigated the efficacy of the cancer-specific CPP carrier BR2 to specifically transport siRNA to cancer-target cells. Our results showed that BR2 formed a complex with anti-vascular endothelial growth factor siRNA (siVEGF) that exhibited the appropriate size and surface charge for in vivo treatment. Additionally, the BR2-VEGF siRNA complex exhibited significant serum stability and high levels of gene-silencing effects in vitro. Moreover, the transfection efficiency of the complex into a cancer cell line was higher than that observed in non-cancer cell lines, resulting in downregulated intracellular VEGF levels in HeLa cellsand comprehensively improved antitumor efficacy in the absence of significant toxicity. These results indicated that BR2 has significant potential for safe, efficient, and specific delivery of siRNA for diverse applications. PMID: 29316746 [PubMed - as supplied by publisher]
Source: Journal of Microbiology and Biotechnology - Category: Biotechnology Authors: Tags: J Microbiol Biotechnol Source Type: research

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U.S. News&World Report ’s newly released ranking of the nation's best children’s hospitals once again placed St. Louis Children’s Hospital in the top 50 in all 10 specialties it analyzes. The 13th annual ranking highlights the top hospitals in 10 pediatric specialties: cancer, cardiology and heart surgery, diabete s and endocrinology, gastroenterology and GI surgery, neonatology, nephrology, neurology and neurosurgery, orthopedics, pulmonology, and urology. St. Louis Children’s Hospital…
Source: bizjournals.com Health Care:Physician Practices headlines - Category: American Health Authors: Source Type: news
Johns Hopkins Children's Center has been ranked as the best pediatric hospital in the state, and once again as one of the 10 best in the country, according to U.S. News&World Report. The annual Best Children’s Hospitals rankings are intended to help families of children with complex and rare conditions find the best medical care. The rankings highlight the top 50 hospital centers in 10 pediatric specialties: cancer, cardiology and heart surgery, diabetes and endocrinology, g astroenterology and…
Source: bizjournals.com Health Care:Physician Practices headlines - Category: American Health Authors: Source Type: news
We report the application of RNA sequencing technology for high-throughput profiling of mRNA expression in breast cancer cell line
Source: GEO: Gene Expression Omnibus - Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Homo sapiens Source Type: research
Contributors : Chaima Cherif ; Julien Vernerey ; Nadine Carbuccia ; Palma Rocchi ; Estelle DuprezSeries Type : Genome binding/occupancy profiling by high throughput sequencingOrganism : Homo sapiensMenin protein is encoded by the multiple endocrine neoplasia type 1 (MEN1) gene. It remains an enigmatic protein, known as a tumor suppressor but its roles in cancer development have attracted significant attention in recent years. The goals of this study is to understand how the Menin tumor suppressor can be oncogenic in Prostate, we have profiled Menin chromatin occupancy by chromatin immunoprecipitation coupled to high-throug...
Source: GEO: Gene Expression Omnibus - Category: Genetics & Stem Cells Tags: Genome binding/occupancy profiling by high throughput sequencing Homo sapiens Source Type: research
Contributors : Manish Aghi ; Jordan SpatzSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensCancer Fibroblasts Isolated from 2 GBM Cases.
Source: GEO: Gene Expression Omnibus - Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Homo sapiens Source Type: research
Contributors : Rebecca A Helmer ; Gurvinder Kaur ; Lisa A Smith ; Beverly S ChiltonSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusThe helicase-like transcription factor (HLTF) gene – a tumor suppressor in human colorectal cancer (CRC) - is regulated by alternative splicing and promoter hypermethylation. The detection of hypermethylated HLTF DNA in fecal occult blood tests is an indicator of disease recurrence and poor survival. Hltf-deficiency in the ApcMin/+ mouse strain in creased the formation of intestinal adenocarcinoma with a high incidence of gross chromosomal instabilit...
Source: GEO: Gene Expression Omnibus - Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Mus musculus Source Type: research
Contributors : Jing Li ; Wei Sun ; Wenge Zhu ; Wei ZhengSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensOvarian cancer IGROV1 cells were studied to profile the expression of wild type (WT, Cisplatin Susceptible) cells and Cisplatin Resistant (CR) cells. The goal was to gain insights or to build hypotheses into the mechanisms of Cisplatin resistance in this cell-based model.
Source: GEO: Gene Expression Omnibus - Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Homo sapiens Source Type: research
Contributors : Grit Pattschull ; Susanne Walz ; Eva Ruehl ; Elmar Wolf ; Carsten P Ade ; Susanne Kneitz ; Andreas Rosenwald ; Bjoern von Eyss ; Stefan GaubatzSeries Type : Genome binding/occupancy profiling by high throughput sequencing ; Expression profiling by high throughput sequencingOrganism : Homo sapiens ; Mus musculusYAP/TAZ, downstream effectors of the Hippo pathway, are important regulators of proliferation. Here we show that the ability of YAP to activate mitotic gene expression is dependent on the Myb-MuvB (MMB) complex, a master regulator of genes expressed in the G2/M phase of the cell cycle. By carrying out ...
Source: GEO: Gene Expression Omnibus - Category: Genetics & Stem Cells Tags: Genome binding/occupancy profiling by high throughput sequencing Expression profiling by high throughput sequencing Homo sapiens Mus musculus Source Type: research
Source: Cancer Management and Research - Category: Cancer & Oncology Tags: Cancer Management and Research Source Type: research
Source: Cancer Management and Research - Category: Cancer & Oncology Tags: Cancer Management and Research Source Type: research
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