Specify genome locations with 30 nucleotides of flanking sequence!
Many of us have had the experience of trying to reconstruct what someone has done and been frustrated trying to find the exact sequence. Relative coordinates do not last: gene models often change so that “Leu234” in a protein is no longer there and our knowledge of genome sequence changes (or we are working with a different strains) so the EcoR1 site 5’ to your favorite gene is not there. There is an easy solution: always specify a location by sequence. Thirty nucleotides is sufficient in essentially all cases to uniquely locate the site. Your simple effort in specifying a genome location by sequence, when you are writing a paper will make experiments easily reproducible, as well as help WormBase in curating such studies.
[TWO CASES OF USING DUPILUMAB FOR REFRACTORY ATOPIC DERMATITIS WHO HAD HISTORIES OF BACTEREMIA AND UNDERLYING CONGENITAL HEART DISEASE]. Arerugi. 2019;68(6):701-706 Authors: Hamada K, Kasai K, Sasaki Y Abstract Defects in the skin's barrier function are known to make it more likely for skin and soft tissue infection to occur in association with atopic dermatitis. These secondary infections sometimes develop into systemic infections such as bacteremia. Here, we report on our use of anti-IL-4/13 monoclonal antibody (dupilumab) on two cases with atopic dermatitis that was refractory to conventional manag...
We report the development of eosinophilic polyangiitis granulomatosis (EGPA) in a young girl being treated for both atopic dermatitis, diagnosed at 1 year of age, and bronchial asthma, diagnosed at 4 years of age. Her eruption did not result in lichenification and was not fully responsive to corticosteroid ointment. Asthma lightened by treatment of inhalational steroids. Hypereosinophilia was detected at 5 years of age, at least 20% of white blood cells, and 44% at 8 years of age. At 10 years of age, she was diagnosed with anti-neutrophil cytoplasmic antibody-negative EGPA. The diagnosis was based on findings of eosinophil...
CONCLUSION: Low albumin and chest radiographic appearance exceeding one-lung area were risk factors for developing PR. Diffuse pulmonary infiltrates in early phase of anti-tuberculosis treatment was related with Inhospital mortality. PMID: 31308335 [PubMed - in process]
CONCLUSIONS: Standardized house dust mite extract was more effective than non-standardized house dust extract for subcutaneous immunotherapy; however, the establishment of safer methods is warranted. PMID: 31308334 [PubMed - in process]
[MUCOSAL MULTI-ECOSYSTEM FOR THE PREVENTION AND TREATMENT OF MUCOSAL DISORDERS]. Arerugi. 2019;68(6):675-680 Authors: Tokuhara D, Kiyono H PMID: 31308333 [PubMed - in process]
[ASTHMA PREVENTION AND MANAGEMENT GUIDELINE 2018 (SECTIONS FOR ADULTS): FOCUSING ON THE TREATMENT OF SEVERE ASTHMA]. Arerugi. 2019;68(6):669-674 Authors: Niimi A PMID: 31308332 [PubMed - in process]
[THE ROLL OF B CELLS IN ALLERGY]. Arerugi. 2019;68(6):661-667 Authors: Saito Y, Baba Y PMID: 31308331 [PubMed - in process]
Publication date: Available online 16 July 2019Source: The SurgeonAuthor(s): Valeria Tonini, Arianna Birindelli, Stefania Bianchini, Maurizio Cervellera, Maria Letizia Bacchi Reggiani, James Wheeler, Salomone Di SaverioAbstractBackgroundThe number of harvested lymph nodes (LNs) in colorectal cancer surgery relates to oncologic radicality and accuracy of staging. In addition, it affects the choice of adjuvant therapy, as well as prognosis. The American Joint Committee on Cancer defines at least 12 LNs harvested as adequate in colorectal cancer resections. Despite the importance of the topic, even in high-volume colorectal c...
ConclusionSimultaneous resection of hepatic and peritoneal metastases seems to provide a survival benefit for selected patients and is associated with acceptable morbidity and mortality rates. Knowledge of patients and operative factors linked to morbidity will help to provide a strict selection process and a safer surgical procedure.