Delivery of Amonafide from Fructose-coated Nanodiamonds by Oxime Ligation for Treatment of Human Breast Cancer.

Delivery of Amonafide from Fructose-coated Nanodiamonds by Oxime Ligation for Treatment of Human Breast Cancer. Biomacromolecules. 2018 Jan 09;: Authors: Zhao J, Lu M, Lai H, Lu H, Lalevée J, Barner-Kowollik C, Stenzel MH, Xiao P Abstract Introducing a strategy towards polymer/nanodiamond hybrids with high polymer grafting density and accessible polymer structural characterisation is of critical importance for nanodiamonds surface modification and bioagent attachment for their biomedical application. Here, we report a glycopolymer/nanodiamond hybrid drug delivery system, which was prepared by grafting amonafide-conjugated glycopolymers onto the surface of nanodiamonds via oxime ligation. Poly(1-O-methacryloyl-2,3:4,5-di-O-isopropylidene-β-D-fructopyranose)-b-poly(3-vinylbenzaldehyde-co-methyl methacrylate), featuring pendant aldehyde groups, is prepared via RAFT polymerization. The anticancer drug amonafide is conjugated to the polymer chains by via imine chemistry, resulting in acid degradable imine linkages. The obtained amonafide-conjugated glycopolymers are subsequently grafted onto the surface of aminooxy-functionalised nanodiamonds via oxime ligation. The molecular weight of the conjugated polymers is characterised by Size Exclusion Chromatography (SEC), while the successful conjugation and corresponding grafting density is characterised by Nuclear Magnetic Resonance (NMR), Fourier transform infrared spectroscopy (FTIR), and ...
Source: Biomacromolecules - Category: Biochemistry Authors: Tags: Biomacromolecules Source Type: research